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Leptin antibody

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Principal name

Leptin antibody

Alternative names for Leptin antibody

LEP, OB, OBS, Obesity factor, Obese protein

SwissProt ID

O02720 (Canfa), O42164 (Chick), P41159 (Human), P41160 (Mouse), P50595 (Bovin), P50596 (Rat), Q28603 (Sheep), Q29406 (Pig), Q8WML2 (Rabit), Q9N2C1 (Felca), Q9TU09 (Horse)

Gene ID

3952 (LEP), 25608 (Lep), 16846

Ncbi ID

NP_000221.1, NP_037208.1, NP_032519.1, 9606, NP_000221, 10090, 10116, NP_032519, NP_037208, NM_000230, NM_013076, NM_008493

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Goat, Sheep, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of Leptin antibody

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be linked to reproductive function.

General readings

1. Ricci MR, Lee MJ, Russell CD, Wang Y, Sullivan S, Schneider SH, Brolin RE, Fried SK . Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am J Physiol Endocrinol Metab . Apr;288(4):E798-804 (2005)
See: http://ajpendo.physiology.org/cgi/content/full/288/4/E798
2. Ricci M.R., Lee M.J., et al. Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am. J. Physiol. Endocrinol. Metab. 2005; (288), E798-E804.
3. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight reduced subjects. Nature Med. 1995; 1155 1161.
4. Maffei M., Halaas J., Ravussin E. et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155-1161 (1995) .
5. Lonnqvist F., Arner P., Nordfors L. and Shalling M. Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950-953 (1995) .
6. Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J. Positional Cloning of the Mouse Obese Gene and its Human Homologue. Nature 372, 425-432 (1994).
7. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal weight and obese humans. N. Engl. J. Med. 1996; (334), 292 295.
8. Considine R.V., Sinha M.K., Heiman M.L. et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292-295 (1996).
9. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight reducing effects of the plasma protein encoded by the obese gene. Science 1995; (269), 543 546.
10. Halaas J.L., Gajiwala K.S., Maffei M. et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995).

AP07406PU-N - Leptin
Breast: Formalin-Fixed Paraffin-Embedded (FFPE)
AP13112PU-N - Leptin
Confocal immunofluorescent analysis of Leptin Antibody (C-term) Cat.-No AP13112PU-N with HepG2 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
AP13112PU-N - Leptin
Western blot analysis of Leptin Antibody (C-term) Cat.-No AP13112PU-N in MDA-MB231 cell line lysates (35ug/lane). LEP (arrow) was detected using the purified Pab.
AP20617PU-N - Leptin
Immunohistochemistry analyzes of Ob antibody (Cat.-No.: AP20617PU-N) in paraffin-embedded human breast carcinoma tissue.
AP20617PU-N - Leptin
Immunohistochemistry (IHC) analyzes of Ob antibody (Cat.-No.: AP20617PU-N) in paraffin-embedded human breast carcinoma tissue.
AP52465PU-N - Leptin
Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Leptin Antibody (N-term) Cat.-No AP52465PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP52465PU-N - Leptin
Confocal immunofluorescent analysis of Leptin Antibody (N-term) Cat.-No AP52465PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
AP52465PU-N - Leptin
Western blot analysis of LEP (arrow) using Leptin Antibody (N-term) Cat.-No AP52465PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.
AP52465PU-N - Leptin
Western blot analysis of Leptin Antibody (N-term) Cat.-No AP52465PU-N in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).
AP52465PU-N - Leptin
Flow cytometric analysis of Hela cells using Leptin Antibody (N-term) Cat.-No AP52465PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ARP41697_P050 - Leptin
Human Placenta; Placenta; LEP antibody - N-terminal region (ARP41697_P050) in Human Placenta cells using Immunohistochemistry
ARP41697_P050 - Leptin
Human Small Intestine; WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Small Intestine; LEP antibody - N-terminal region (ARP41697_P050) in Human Small Intestine cells using Western Blot
ARP41698_P050 - Leptin
Human HepG2; WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. LEP is supported by BioGPS gene expression data to be expressed in HepG2.; LEP antibody - middle region (ARP41698_P050) in Human HepG2 cells using Western Blot
H00003952-M01 - Leptin
Detection limit for recombinant GST tagged LEP is approximately 0.1ng/ml as a capture antibody.
H00003952-M02 - Leptin
Detection limit for recombinant GST tagged LEP is approximately 0.1ng/ml as a capture antibody.
PAB9544 - Leptin
Western blot analysis with recombinant human LEP.Lane 1 : 10 ng.Lane 2 : 50 ng.
PAB9545 - Leptin
Western blot analysis of recombinat Lep.Lane 1 : 10 ng.Lane 2 : 50 ng.
PP050P1 - Leptin
PP050P1 - Leptin
Immunohistochemistry The Leptin antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 μg/mL overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania.
PP1041B1 - Leptin
Direct ELISA using Biotin conjuagted Leptin Antibody Cat.-No PP1041B
PP1041B1 - Leptin
Sandwich ELISA using Biotin conjuagted Leptin Antibody Cat.-No PP1041B
AR50000PU-N - Leptin
AR50010PU-N - Leptin
CEK1254 - Human Leptin ELISA Kit
Representative Standard Curve
CEK1255 - Human Leptin ELISA Kit
Representative Standard Curve
TP309259 - Leptin
TP720054 - Leptin
TP723267 - Leptin
TP723268 - Leptin
TP723269 - Leptin
TA321089 - Leptin
Predicted cell location: Cytoplasm. Positive control: Human thyroid and cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
TA321089 - Leptin
Predicted cell location: Cytoplasm. Positive control: Human thyroid and cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using LEP antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
TA346994 - Leptin
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml.
TA346995 - Leptin
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml.
TA319080 - Leptin
Western blot analysis using recombinant rat Leptin. Lane 1: 5 ng, Lane 2: 10 ng.
TA321088 - Leptin
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200)
TA321088 - Leptin
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using LEP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
TA328366 - Leptin
To detect hLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin.
TA328366 - Leptin
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Leptin (TA328367) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin.
TA328367 - Leptin
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.
TA328367 - Leptin
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.
TA328367 - Leptin
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.
TA328528 - Leptin
To detect mLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin.
TA328528 - Leptin
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Leptin (TA328529) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin.
TA328529 - Leptin
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
TA328529 - Leptin
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
TA328529 - Leptin
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
TA332832 - Leptin
Western blot analysis of extracts of various cell lines, using LEP antibody.
TA350133 - Leptin
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (LEP Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Leptin (N-term) antibody
Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Leptin Antibody (N-term) Cat.-No AP52465PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+4 additional images

Highly validated antibody!

Leptin antibody
  Rabbit polyclonal anti-Leptin antibody  
Western blot analysis using recombinant rat Leptin. Lane 1: 5 ng, Lane 2: 10 ng. Rabbit Purified Rt WB
0.1 mg / €355.00
  OriGene Technologies, Inc.

Leptin antibody
  Anti-LEP Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Leptin antibody
  Anti-LEP Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid and cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using LEP antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Leptin antibody
  Biotinylated Anti-Human Leptin Rabbit Polyclonal Antibody  
To detect hLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, WB
50 µg / €275.00
  OriGene Technologies, Inc.
+1 additional image

Leptin antibody
  Anti-Human Leptin Rabbit Polyclonal Antibody  
To detect hLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin (TA328366) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+2 additional images

Leptin antibody
  Biotinylated Anti-Murine Leptin Rabbit Polyclonal Antibody  
To detect mLeptin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, WB
50 µg / €275.00
  OriGene Technologies, Inc.
+1 additional image

Leptin antibody
  Anti-Murine Leptin Rabbit Polyclonal Antibody  
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (TA328528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin. Rabbit IgG Purified E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+2 additional images

Leptin antibody
  Rabbit anti-LEP Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using LEP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

Leptin antibody
  Mouse Monoclonal Leptin Antibody  
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG2a 5C11-G6-B12 Purified
0.1 ml / €335.00
  OriGene Technologies, Inc.

Leptin antibody
  Mouse Monoclonal Leptin Antibody  
Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml. Mouse IgG1 7E3-D11-B7 Purified
0.1 ml / €335.00
  OriGene Technologies, Inc.

Leptin antibody
  Rabbit Polyclonal Anti-LEP Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (LEP Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

Leptin antibody
Leptin Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Goat Biotin Rt E, WB
50 µg / €340.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Goat Biotin Rt E, WB
25 µg / €250.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Goat Aff - Purified Rt E, WB
0.1 mg / €340.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Goat Aff - Purified Rt E, WB
50 µg / €250.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Sheep IgG Purified Hu, Ms, Rt E
1 ml / €410.00
  OriGene Technologies GmbH

Leptin antibody
Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Serum Hu, Ms E, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

Leptin antibody
Leptin Rabbit IgG Ig Fraction Ms E, WB
1 mg / €1,345.00
  OriGene Technologies GmbH

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53 products - FILTER YOUR RESULTS!

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