Leukemia inhibitory factor antibody
Principal name
Leukemia inhibitory factor antibody
Alternative names for Leukemia inhibitory factor antibody
LIF, HILDA, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Differentiation-stimulating factor
Ncbi ID
NP_002300.1, NP_032527.1, 10116, 9606, NP_032527, NP_002300, AAA37211.1, NM_002309, NM_001039537
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Leukemia inhibitory factor antibody
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neurol cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the materl-fetal interface.Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF sigls through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.
Western Blot analysis of LIF expression in transfected 293T cell line (H00003976-T01) by LIF MaxPab polyclonal antibody.Lane 1: LIF transfected lysate(22.22 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of LIF Antibody (Center) (AP17537PU-N) in CEM cell line lysates (35ug/lane). LIF (arrow) was detected using the purified Pab.
Human Muscle; WB Suggested Anti-LIF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; LIF antibody - N-terminal region (ARP54690_P050) in Human Muscle cells using Western Blot
Staining of Mouse Colon lysate using LIF Antibody AP32068PU-N at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of LIF expression in Jurkat (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Western blot analysis using recombinant human LIF. Lane 1: 100 ng, Lane 2: 200 ng, Lane 3: 500 ng.
Western blot analysis of LIF in 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.
Immunofluorescence of LIF in 3T3 cells with LIF antibody at 20 ug/mL.
Predicted cell location: Cytoplasm. Positive control: Human stomach and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human stomach and thyroid cancer tissue using LIF antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human stomach and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human stomach and thyroid cancer tissue using LIF antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-LIF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Muscle
Western Blot: LIF Antibody (39N7D10) [TA337172] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and © human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-ra
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as t
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts deve
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunepositivity of LIF was observ
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepos
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [TA337172] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic imm
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337172 | Leukemia inhibitory factor antibody |
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| Rat Monoclonal LIF Antibody (39N7D10) | |||||||
![Western Blot: LIF Antibody (39N7D10) [TA337172] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and © human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-ra](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-27406-1-W.jpg) |
Rat | IgG2b | 39N7D10 | Purified | Hu, Ms | P, WB |
0.1 mg /
€395.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| AP32068PU-N | Leukemia inhibitory factor (28-39) antibody |
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Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€380.00
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA319047 | Leukemia inhibitory factor antibody |
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| Rabbit polyclonal anti-leukemia inhibitory factor (LIF) antibody | |||||||
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Rabbit | Purified | Hu | WB |
0.1 mg /
€355.00
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| OriGene Technologies, Inc. | |||||||
| TA319675 | Leukemia inhibitory factor (Center) antibody |
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| Rabbit Polyclonal LIF Antibody | |||||||
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Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321468 | Leukemia inhibitory factor antibody |
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| Anti-LIF Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| DM3587P | Leukemia inhibitory factor antibody |
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Rat | IgG2 | 5M28 | Purified | Ms | WB |
0.1 mg /
€460.00
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| OriGene Technologies GmbH | |||||||
| DP3523 | Leukemia inhibitory factor antibody |
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Rabbit | Purified | Hu | WB |
0.2 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| DP3523S | Leukemia inhibitory factor antibody |
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Rabbit | Purified | Hu | WB |
0.1 mg /
€270.00
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| OriGene Technologies GmbH | |||||||
| DP3525 | Leukemia inhibitory factor antibody |
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Rabbit | Purified | Ms | WB |
0.2 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| DP3525S | Leukemia inhibitory factor antibody |
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Rabbit | Purified | Hu | WB |
0.1 mg /
€270.00
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| OriGene Technologies GmbH | |||||||
| MO16101-100 | Leukemia inhibitory factor antibody |
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Mouse | IgG1 | 4F7.2 | Purified | Hu | WB |
0.1 mg /
€580.00
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| Neuromics Antibodies | |||||||
| MO16102-100 | Leukemia inhibitory factor antibody |
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Mouse | IgG1 | 2H2.2 | Purified | Ms | WB |
0.1 mg /
€570.00
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| Neuromics Antibodies | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN309278 | Lif - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN309278BN | Lif - mouse gene knockout kit via CRISPR |
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Mouse | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN309278LP | Lif - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN309278RB | Lif - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310818 | Leukemia inhibitory factor |
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| Recombinant protein of human leukemia inhibitory factor (cholinergic differentiation factor) (LIF) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
| TP720015 | Leukemia inhibitory factor |
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| Recombinant protein of human leukemia inhibitory factor (cholinergic differentiation factor) (LIF) | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
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| OriGene Technologies, Inc. | |||||
| TP723270 | Leukemia inhibitory factor |
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| Purified recombinant protein of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF). | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
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| OriGene Technologies, Inc. | |||||
| TP723889 | Leukemia inhibitory factor |
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| Purified recombinant protein of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF) | |||||
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Human | > 97 % Preparation: . Purity Detail: >97%, as determined by Coomassie stained SDS-PAGE. |
E. coli |
10 µg /
€190.00
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| OriGene Technologies, Inc. | |||||
