LIMS1 antibody
Principal name
LIMS1 antibody
Alternative names for LIMS1 antibody
LIMS-1, PINCH, PINCH1, Particularly interesting new Cys-His protein 1, PINCH-1, Renal carcinoma antigen NY-REN-48, LIM and senescent cell antigen-like-containing domain protein 1
Ncbi ID
9606, NP_001180417.1, NM_004987, NM_001193482, NM_001193484, NM_001193485, NP_080424.2, NM_026148, NM_201242, NM_001193303, NM_001193483
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of LIMS1 antibody
- Kit Component:
- - KN309294G1, Lims1 gRNA vector 1 in pCas-Guide vector
- KN309294G2, Lims1 gRNA vector 2 in pCas-Guide vector
- KN309294D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);
Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
WB Suggested Anti-LIMS1 Antibody; Titration: 1.0 ug/ml; Positive Control: U937 Whole Cell
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human kidney tissue, mouse lung tissue, Primary antibody: (LIMS1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (LIMS1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (LIMS1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06652SU-N | LIMS1 antibody |
||||||
 |
Mouse | IgG1 | 5G7 | Ascites | Hu | E, ICC/IF, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
||||||
| TA350137 | LIMS1 antibody |
||||||
| Rabbit Polyclonal Anti-LIMS1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417616 | LIMS1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY434163 | LIMS1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY434184 | LIMS1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY434199 | LIMS1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205021L2V | Lenti ORF particles, Lims1 (GFP-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205021L1V | Lenti ORF particles, Lims1 (Myc-DDK-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||