LIN28 antibody
Principal name
LIN28 antibody
Alternative names for LIN28 antibody
LIN-28, LIN28A, CSDD1, ZCCHC1, Lin-28 homolog A
Ncbi ID
9606, NP_078950, NP_078950.1, NM_024674, EAX07814, NP_665832.1, NM_145833
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C)
Background of LIN28 antibody
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
General readings
Peng, S., et al. Oncogene 29(14):2153-2159(2010)
Qiu, C., et al. Nucleic Acids Res. 38(4):1240-1248(2010)
Iliopoulos, D., et al. Cell 139(4):693-706(2009)
Heo, I., et al. Cell 138(4):696-708(2009)
West, J.A., et al. Nature 460(7257):909-913(2009)
Immunofluorescence of purified MaxPab antibody to LIN28 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of LIN28 expression in transfected 293T cell line (H00079727-T01) by LIN28 MaxPab polyclonal antibody.Lane 1: LIN28 transfected lysate(23.1 KDa).Lane 2: Non-transfected lysate.
Figure 2. Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#AM11048PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Figure 1. Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody (Cat.#AM11048PU-N). LIN28 (arrow) was detected using the purified Mab.
Recombinant human Lin28 (42-209 aa), His-tagged: 15% SDS-PAGE (3 µg)
Human Testis; Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-LIN28 antibody (ARP34477_P050); LIN28 antibody - N-terminal region (ARP34477_P050) in Human Testis cells using Immunohistochemistry
Human HeLa; WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; LIN28 antibody - N-terminal region (ARP34477_P050) in Human HeLa cells using Western Blot
Western blot analysis of extracts from HepG2 cells using Lin28 Antibody AP26066PU-N.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of LIN28 Antibody Cat.-No AP52486PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the LIN28 antibody detected the LIN28 protein (arrow).
Western blot analysis of LIN28 Antibody Cat.-No AP52486PU-N in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the LIN28 antibody detected the LIN28 protein (arrow).
Flow cytometric analysis of MDA-MB435 cells using LIN28 Antibody Cat.-No AP52486PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 antibody Cat.-No AM06277PU-N (green). Blue: DRAQ5 fluorescent DNA dye.
Confocal Immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of Human LIN28, cells were analyzed ~62 hours after transfection.
Western blot analysis using LIN28 antibody Cat.-No AM06277PU-N against NTERA-2 cell lysate (Lane 1).
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN28A (RC203397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN28A.
ELISA: LIN28 Antibody
Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of KLF4 in human spleen tissue with KLF4 antibody at 5 ug/mL.
Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 ug/mL.
Predicted band size: 23 kDa . Positive control: HepG2 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
LIN28A Antibody (Center) (Cat. #TA324388) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).
LIN28A Antibody (Center) (Cat. #TA324388)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIN28A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of SY5Y cells stained with TA324388 LIN28A (Center) antibody. SY5Y cells were incubated with TA324388 LIN28A (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Immunofluorescent analysis of A549 cells, using LIN28A Antibody (Center) (Cat. #TA324388). TA324388 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
LIN28A Antibody (Center) (Cat. #TA324388) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0 ug/ml using anti-LIN28 antibody
Western Blot: Lin28 Antibody [TA336788] - Analysis of LIN28 in NTERA-2 cell lysate
Immunohistochemistry: Lin28 Antibody [TA336788] - Immunohistochemical analysis of LIN28 in E17.5 mouse embryo
Immunocytochemistry/Immunofluorescence: Lin28 Antibody [TA336788] - Analysis of LIN28 in NTERA-2 cells using TA336788. Nuclei (Blue) are counterstained using Hoechst 33258.
Western blot detection of LIN28 in NTERA and F9 cell lysates using LIN28A mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.
Western blot detection of LIN28A in LIN28B and LIN28A recombinant antigen fragments and using LIN28A mouse mAb (1:1000 diluted).
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (LIN28A Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324388 | LIN28 (Center) antibody |
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| Rabbit polyclonal LIN28A Antibody (Center) | |||||||
 |
Rabbit | Ig | Purified | Hu, Ms | F, P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA306924 | LIN28 antibody |
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| Rabbit Polyclonal Lin28 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA322179 | LIN28 antibody |
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| Anti-LIN28A Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326716 | LIN28 antibody |
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| Mouse Monoclonal Lin28 Monoclonal Antibody | |||||||
 |
Mouse | IgG2b | 1G9H9 | Purified | Hu | WB |
0.1 mg /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| TA336788 | LIN28 antibody |
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| Rabbit Polyclonal LIN-28A Antibody | |||||||
![Western Blot: Lin28 Antibody [TA336788] - Analysis of LIN28 in NTERA-2 cell lysate](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-49537-1-W.jpg) |
Rabbit | Purified | Hu, Ms | C, ICC/IF, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA346996 | LIN28 antibody |
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| Mouse Monoclonal LIN28A Antibody | |||||||
 |
Mouse | IgG2a | 2C1-F9-A2 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA350754 | LIN28 antibody |
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| Rabbit Polyclonal Anti-LIN28A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06277PU-N | LIN28 antibody |
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 |
Mouse | IgG1 | 6D1F9 | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€420.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AM11048PU-N | LIN28 antibody |
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 |
Mouse | IgG1 | 55CT58.12.1 | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP23938PU-N | LIN28 (C-term) antibody |
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 |
Rabbit | Purified | Hu | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP26066PU-N | LIN28 (1-5) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
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| OriGene Technologies GmbH | |||||||
| AP26066PU-S | LIN28 (1-5) antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| AP52486PU-N | LIN28 antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203397 | LIN28A - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203397BN | LIN28A - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203397LP | LIN28A - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203397RB | LIN28A - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303397 | LIN28 |
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| Purified recombinant protein of Homo sapiens lin-28 homolog (C. elegans) (LIN28) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723273 | LIN28 |
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| Purified recombinant protein of Human lin-28 homolog A (C. elegans) (LIN28A). | |||||
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Human | > 90 % Preparation: . Purity Detail: >90% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411135 | LIN28A overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |