LIN28B antibody
Principal name
LIN28B antibody
Alternative names for LIN28B antibody
LIN28B, CSDD2, Lin-28 homolog B
SwissProt ID
Q6ZN17 (Human)
Gene ID
389421 (LIN28B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of LIN28B antibody
Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
General readings
Heo I., et al. (2008) Mol. Cell 32:276-284Richards M, et al. (2004) Stem Cells 22 (1): 51–64.
Western blot analysis of LIN28B polyclonal antibody ( Cat # PAB3152 ) in HL-60 cell line lysates ( 35 µg/lane ) . LIN28B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of LIN28B polyclonal antibody ( Cat # PAB3153 ) in HL-60 cell line lysates ( 35 µg/lane ) . LIN28B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28B polyclonal antibody ( Cat # PAB3154 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of LIN28B polyclonal antibody ( Cat # PAB3154 ) in K-562 cell line lysates ( 35 µg/lane ) . LIN28B ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of LIN28B Antibody (C-term) in HL60 cell line lysates (35 µg/lane). LIN28B (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with LIN28B antibody (Center) (Cat.-No AP11496PU-N), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes : Anti-LIN28B Antibody (Center) at 1/1000 dilution. Lane 1: HepG2 whole cell lysates, Lane 2: K562 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LIN28B Antibody (Center) at 1/2000 dilution. Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: mouse testis lysate, Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28B antibody (Center) (Cat.-No AP11496PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of LIN28B antibody (Center) (Cat.-No AP11496PU-N) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-LIN28B antibody (Cat.-No AP11496PU-N) in K562 cell line lysates (35 µg/lane). LIN28B (arrow) was detected using the purified Pab (1:60 dilution).
Flow Cytometric analysis of HL-60 cells using LIN28B antibody (Center) (Cat.-No AP11496PU-N) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Human Spleen (formalin-fixed, paraffin-embedded) stained with LIN28B antibody AP22769PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Testis (formalin-fixed, paraffin-embedded) stained with LIN28B antibody AP22769PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of LIN28B in HL60 cell line lysates (35 µg/lane).
ELISA: LIN28B Antibody
Predicted band size: 27 kDa . Positive control: Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Hela and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of LIN28B Antibody (N-term) (Cat.#TA324574) in HL60 cell line lysates (35ug/lane). LIN28B (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human Testis tissue reacted with LIN28B Antibody (N-term)(Cat.#TA324574), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of SY5Y cells stained with LIN28B (N-term) antibody. SY5Y cells were incubated with TA324574 LIN28B (N-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).
Flow cytometric analysis of HL-60 cells using LIN28B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts from Hela and 293 cells using LIN28B Antibody
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, 293T cells, Primary antibody: (LIN28B Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP11496PU-N | LIN28B antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | F, ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA323503 | LIN28B antibody |
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| Anti-LIN28B Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA324574 | LIN28B (N-term) antibody |
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| Rabbit polyclonal LIN28B Antibody (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA347832 | LIN28B antibody |
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| Rabbit Polyclonal Anti-LIN28B Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| TA350898 | LIN28B antibody |
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| Rabbit Polyclonal Anti-LIN28B Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| TA590769 | LIN28B antibody |
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| Rabbit Polyclonal Lin28B Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
50 µg /
€409.00
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| OriGene Technologies, Inc. | |||||||
| AP11495PU-N | LIN28B (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| AP22769PU-N | LIN28B (N-term, 1-30) antibody |
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 |
Rabbit | Purified | Hu | F, ICC/IF, P, WB |
0.2 ml /
€425.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760648 | LIN28B (full length, N-term HIS tag) |
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| Purified recombinant protein of Human lin-28 homolog B (C. elegans) (LIN28B), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424091 | LIN28B overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. |