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LMX1B antibody

Principal name

LMX1B antibody

Alternative names for LMX1B antibody

LMX1B, LIM homeobox transcription factor 1-beta, LIM/homeobox protein LMX1B, LIM/homeobox protein 1.2, LMX-1.2

SwissProt ID

O60663 (Human), O88609 (Mouse), P53413 (Chick), Q60564 (Mesau)

Gene ID

4010 (LMX1B)

Ncbi ID

9606, NP_002307, NP_001167618.1, NP_001167617, NM_002316, NM_001174147

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Por (Porcine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of LMX1B antibody

Kit Component:: - KN210188G1, LMX1B gRNA vector 1 in pCas-Guide vector
- KN210188G2, LMX1B gRNA vector 2 in pCas-Guide vector
- KN210188D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83.
Nakatani T, Kumai M, Mizuhara E, et al. Lmx1a and Lmx1b cooperate with Foxa2 to coordinate the specification of dopaminergic neurons and control of floor plate cell differentiation in the developing mesencephalon. Dev. Biol. 2010; 339:101-13.
Guo C, Qiu HY, Huang Y, et al. Lmx1b is essential for FGF8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice. Development 2007; 134:317-25.
Chizhikov VV and Millen KJ. Control of roof plate formation by LMX1A in the developing spinal cord. Development 2004; 131:2693-705.

Human Muscle; WB Suggested Anti-LMX1B Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; LMX1B antibody - C-terminal region (ARP38140_T100) in Human Muscle cells using Western Blot

ARP38140_T100

Immunoperoxidase of monoclonal antibody to LMX1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

H00004010-M08

Detection limit for recombinant GST tagged LMX1B is approximately 0.3ng/ml as a capture antibody.

H00004010-M08

Western blot (WB) analysis of LMX1B antibody (Cat.-No.: AP20515PU-N) in extracts from hela cells.

AP20515PU-N

Western blot analysis of extracts from Jurkat/293/HuvEc cells, using LMX1B Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of LMX1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of LMX1B expression in HeLa (A); Jurkat (B); SP2/0 (C) whole cell lysates.

Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using LMX1B antibody.The lane on the right is treated with the synthesized peptide.

TA314159

Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 µg/mL.

TA319063

Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 ug/mL.

TA319677

Immunohistochemistry of LMX1B in human brain tissue with LMX1B antibody at 5 ug/mL.

TA319677

Immunofluorescence of LMX1B in human brain tissue with LMX1B antibody at 20 ug/mL.

TA319677

WB Suggested Anti-LMX1B Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle

TA335840

Western blot analysis on Jurkat cell lysate using LMX1B Antibody

TA347546

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA314159	LMX1B antibody
	Rabbit polyclonal anti-LMX1B antibody
	Rabbit	IgG	Purified	Hu, Ms	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA319063	LMX1B antibody
	Rabbit polyclonal anti-LMX1B antibody (CT)
	Rabbit		Purified	Hu, Ms, Rt	E, WB	0.1 mg / €450.00
						OriGene Technologies, Inc.
TA319677	LMX1B (C-term) antibody
	Rabbit Polyclonal LMX1B Antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	E, ICC/IF, WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA347546	LMX1B antibody
	Rabbit Polyclonal Anti-LMX1B Antibody
	Rabbit	IgG	Purified	Hu, Ms	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
AP20515PU-N	LMX1B antibody
	Rabbit		Aff - Purified	Hu, Ms	P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH