LPCAT1 antibody
Principal name
LPCAT1 antibody
Alternative names for LPCAT1 antibody
LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, LysoPC acyltransferase 1, LPC acyltransferase 1, LPCAT-1
Ncbi ID
NP_079106, AAI40368.1, NM_024830, NP_079106.3, NP_663351.3, NM_145376, NM_001100735
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of LPCAT1 antibody
- Kit Component:
- - KN220083G1, LPCAT1 gRNA vector 1 in pCas-Guide vector
- KN220083G2, LPCAT1 gRNA vector 2 in pCas-Guide vector
- KN220083D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Yamazaki T., et al. (2012) Biol Pharm Bull. 35(9):1509-15.Kazachkov M., et al. (2001) Lipids. 43(10):895-902.
Human Small Intestine; WB Suggested Anti-LPCAT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Small Intestine; LPCAT1 antibody - middle region (ARP49884_P050) in Human Small Intestine cells using Western Blot
Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PCAT1 Antibody (C-term) (Cat.#AP53189PU-N) in Hela cell line lysates (35µg/lane). PCAT1 (arrow) was detected using the purified Pab.
PCAT1 Antibody (C-term) (Cat. #AP53189PU-N) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-LPCAT1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LPCAT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-LPCAT1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB Suggested Anti-LPCAT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Small Intestine
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316969 | LPCAT1 antibody |
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| LPCAT1 / AYTL2 Rabbit Polyclonal (Internal) Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA316970 | LPCAT1 antibody |
||||||
| LPCAT1 / AYTL2 Rabbit Polyclonal (Internal) Antibody | |||||||
|
Rabbit | IgG | Aff - Purified | Hu | P |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP53189PU-N | LPCAT1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320083 | LPCAT1 |
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| Recombinant protein of human lysophosphatidylcholine acyltransferase 1 (LPCAT1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403037 | LPCAT1 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203122L2V | Lenti ORF particles, Lpcat1 (GFP-tagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,230.00
|
|
| OriGene Technologies, Inc. | ||
| RR203122L1V | Lenti ORF particles, Lpcat1 (Myc-DDK-tagged ORF) - Rat lysophosphatidylcholine acyltransferase 1 (Lpcat1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||