LRRC57 antibody
Principal name
LRRC57 antibody
Alternative names for LRRC57 antibody
Leucine-rich repeat-containing protein 57
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of LRRC57 antibody
LRRC57 (leucine rich repeat containing 57) is a 239 amino acid protein that contains eight LRR repeats and is encoded by a gene that maps to human chromosome 15q15.2. Chromosome 15 houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.
General readings
Gerhard,D.S., Genome Res. 14 (10B), 2121-2127 (2004)
Western Blot analysis of LRRC57 expression in transfected 293T cell line (H00255252-T01) by LRRC57 MaxPab polyclonal antibody.Lane 1: LRRC57 transfected lysate(26.29 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-LRRC57 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; LRRC57 antibody - middle region (ARP55578_P050) in Human MCF-7 cells using Western Blot
Transfected 293T; WB Suggested Anti-LRRC57 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; LRRC57 antibody - N-terminal region (ARP55577_P050) in Transfected 293T cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma reacted with LRRC57 Antibody (C-term) Cat.-No AP52542PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of LRRC57 Antibody (C-term) Cat.-No AP52542PU-N in 293 cell line lysates (35ug/lane). This demonstrates the LRRC57 antibody detected the LRRC57 protein (arrow).
Flow cytometric analysis of 293 cells using LRRC57 Antibody (C-term) Cat.-No AP52542PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-LRRC57 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
WB Suggested Anti-LRRC57 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: HT29 cells, Primary antibody: (LRRC57 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (LRRC57 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (LRRC57 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350143 | LRRC57 antibody |
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| Rabbit Polyclonal Anti-LRRC57 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP52542PU-N | LRRC57 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308959 | LRRC57 |
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| Recombinant protein of human leucine rich repeat containing 57 (LRRC57) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215368L2V | Lenti ORF particles, Lrrc57 (GFP-tagged ORF) - Rat leucine rich repeat containing 57 (Lrrc57), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR215368L1V | Lenti ORF particles, Lrrc57 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 57 (Lrrc57), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
