LRWD1 antibody
Principal name
LRWD1 antibody
Alternative names for LRWD1 antibody
ORC-associated protein, ORCA, Leucine-rich repeat and WD repeat-containing protein 1, Origin recognition complex-associated protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of LRWD1 antibody
- Kit Component:
- - KN309523G1, 1200011O22Rik gRNA vector 1 in pCas-Guide vector
- KN309523G2, 1200011O22Rik gRNA vector 2 in pCas-Guide vector
- KN309523D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Teng, Y.N., et al. Int. J. Androl. 33(6):832-840(2010)
Shen, Z., et al. Mol. Cell 40(1):99-111(2010)
Vermeulen, M., et al. Cell 142(6):967-980(2010)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with LRWD1 Antibody (N-term) Cat.-No AP52552PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of LRWD1 Antibody (N-term) Cat.-No AP52552PU-N in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the LRWD1 antibody detected the LRWD1 protein (arrow).
Host: Rabbit; Target Name: LOC304396; Sample Tissue: Rat Kidney lysates; Antibody Dilution: 1.0ug/ml
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human LRWD1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:24,900.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52552PU-N | LRWD1 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307141 | LRWD1 |
||||
| Purified recombinant protein of Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY407257 | LRWD1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209749L2V | Lenti ORF particles, Lrwd1 (GFP-tagged) - Mouse leucine-rich repeats and WD repeat domain containing 1 (Lrwd1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,400.00
|
|
| OriGene Technologies, Inc. | ||
| MR209749L1V | Lenti ORF particles, Lrwd1 (Myc-DDK-tagged) - Mouse leucine-rich repeats and WD repeat domain containing 1 (Lrwd1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
