LSM1 antibody
Principal name
LSM1 antibody
Alternative names for LSM1 antibody
CASM, Cancer-associated Sm-like, U6 snRNA-associated Sm-like protein LSm1, Small nuclear ribonuclear CaSm
Ncbi ID
NP_055277, 9606, NP_055277.1, NM_014462, NM_026032, NP_080308.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of LSM1 antibody
- Kit Component:
- - KN200288G1, LSM1 gRNA vector 1 in pCas-Guide vector
- KN200288G2, LSM1 gRNA vector 2 in pCas-Guide vector
- KN200288D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Noueiry A O., et al. (2003) Mol Cell Biol. 23:4094-4106.
Kufel J., et al. (2003) J Biol Chem. 278:2147-2156.
Western blot analysis of anti-LSM1 Antibody (C-term) (Cat.#AP12529PU-N) in Hela cell line lysates (35ug/lane). LSM1(arrow) was detected using the purified Pab.
Western blot analysis of anti-LSM1 Antibody (Center) (Cat.#AP12530PU-N) in mouse kidney tissue lysates (35ug/lane). LSM1 (arrow) was detected using the purified Pab.
NCI-H226; WB Suggested Anti-LSM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: NCI-H226 cell lysate; LSM1 antibody - middle region (ARP40770_P050) in NCI-H226 cells using Western Blot
Recombinant human LSM1 (1-133), His-tagged
Western blot analysis of LSM1 expression in Hela (A); HEK293T (B) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-LSM1 monoclonal antibody.(1:200)
Anti-LSM1 mouse monoclonal antibody (TA503106) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells transfected with either RC200288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LSM1 antibody(TA503106), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-LSM1 antibody(TA503106),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-LSM1 antibody(TA503106),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-LSM1 mouse monoclonal antibody (TA503107) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells transfected with either RC200288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LSM1 antibody(TA503107), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LSM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503120)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LSM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503120)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LSM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503120)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LSM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503120)
Anti-LSM1 mouse monoclonal antibody (TA503120) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LSM1 monoclonal antibody.
HEK293T cells transfected with either RC200288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LSM1 antibody(TA503120), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-LSM1 antibody(TA503120),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-LSM1 antibody(TA503120),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-LSM1 mouse monoclonal antibody (TA503121) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
HEK293T cells transfected with either RC200288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LSM1 antibody(TA503121), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-LSM1 antibody(TA503121),(Red), compared to a nonspecific negative control antibody,(Blue).
WB Suggested Anti-LSM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: NCI-H226 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720235 | LSM1 |
||||
| Recombinant protein of human LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM1) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
