LSM5 antibody
Principal name
LSM5 antibody
Alternative names for LSM5 antibody
U6 snRNA-associated Sm-like protein LSm5
Gene ID
23658 (LSM5)
Ncbi ID
NP_001124182, NP_036454, NP_001124182.1, NM_012322, NM_001130710
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of LSM5 antibody
- Kit Component:
- - KN203266G1, LSM5 gRNA vector 1 in pCas-Guide vector
- KN203266G2, LSM5 gRNA vector 2 in pCas-Guide vector
- KN203266D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Salgado-Garrido J., et al. (1999) EMBO J. 18: 3451-3462.Achsel T., et al. (1999) EMBO J. 18: 5789-5802.Friesen WJ., et al. (2000) J Biol Chem. 275: 26370-26375.Eystathioy T., et al. (2002) Arthritis Rheum. 46: 726-734.
WB Suggested Anti-LSM5 Antibody; Titration: 1.0 ug/ml; Positive Control: MCF7 Whole CellLSM5 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LSM5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : K562 cell lysateLane 3. : Jurkat cell lysate
ICC/IF analysis of LSM5 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LSM5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM50645PU-N | LSM5 antibody |
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 |
Mouse | IgG2a | AT25F8 | Purified | Hu | E, F, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AM50645PU-S | LSM5 antibody |
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|
Mouse | IgG2a | AT25F8 | Purified | Hu | E, F, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303266 | LSM5 (transcript variant 1) |
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| Recombinant protein of human LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM5), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||