Lumican antibody
Principal name
Lumican antibody
Alternative names for Lumican antibody
LUM, LDC, SLRR2D, KSPG lumican, Keratan sulfate proteoglycan lumican
SwissProt ID
O46379 (Rabit), P51884 (Human), P51885 (Mouse), P51886 (Rat), P51890 (Chick), Q05443 (Bovin), Q9TTB4 (Pig)
Ncbi ID
9606, NP_002336.1, NP_002336, NP_032550.2, NP_112312.1, NM_002345, NM_008524, NM_031050
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Lumican antibody
- Kit Component:
- - KN309562G1, Lum gRNA vector 1 in pCas-Guide vector
- KN309562G2, Lum gRNA vector 2 in pCas-Guide vector
- KN309562D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chakravarti S, Stallings RL, et al. (1995). Genomics. 27(3):481-8.
Western Blot analysis of LUM expression in transfected 293T cell line by LUM MaxPab polyclonal antibody.Lane 1: LUM transfected lysate(37.18 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of LUM expression in transfected 293T cell line (H00004060-T02) by LUM MaxPab polyclonal antibody.Lane 1: LUM transfected lysate(38.40 KDa).Lane 2: Non-transfected lysate.
Human Heart; WB Suggested Anti-LUM Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; LUM antibody - middle region (ARP54695_P050) in Human Heart cells using Western Blot
Representative Standard Curve
Representative Standard Curve
Representative Standard Curve
Western blot analysis of extracts of various cell lines, using LTBR antibody.
WB Suggested Anti-LUM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human heart
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Jurkat cells, Primary antibody: (LUM Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (LUM Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (LUM Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332630 | Lumican antibody |
||||||
| Rabbit anti-LTBR Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350145 | Lumican antibody |
||||||
| Rabbit Polyclonal Anti-LUM Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN309562BN | Lum - mouse gene knockout kit via CRISPR |
||
 |
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309562LP | Lum - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309562RB | Lum - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302757 | Lumican |
||||
| Recombinant protein of human lumican (LUM) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720321 | Lumican |
||||
| Recombinant protein of human lumican (LUM) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100737 | Human Lumican ELISA Kit |
|
|
1 kit /
€500.00
|
|
| OriGene Technologies, Inc. | ||
| EA100748 | Mouse Lumican ELISA Kit |
|
|
1 kit /
€500.00
|
|
| OriGene Technologies, Inc. | ||
| EA100749 | Rat Lumican ELISA Kit |
|
|
1 kit /
€500.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY419389 | LUM overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205048L2V | Lenti ORF particles, Lum (GFP-tagged) - Mouse lumican (Lum), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205048L1V | Lenti ORF particles, Lum (Myc-DDK-tagged) - Mouse lumican (Lum), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||