MAD2L1 antibody
Principal name
MAD2L1 antibody
Alternative names for MAD2L1 antibody
Mitotic spindle assembly checkpoint protein MAD2A, MAD2
Ncbi ID
9606, NP_002349.1, NP_002349, NP_062372.2, NM_019499, NM_002358
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of MAD2L1 antibody
- Kit Component:
- - KN309637G1, Mad2l1 gRNA vector 1 in pCas-Guide vector
- KN309637G2, Mad2l1 gRNA vector 2 in pCas-Guide vector
- KN309637D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Wei, J. H., et al., J. Biol. Chem. 280, 7748-7757 (2005).
2. Sudakin, V., et al., J. Cell Biol. 154, 925-936 (2001).
Clone 2G9 is used in reference number 1).
MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Western Blot analysis of MAD2L1 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of MAD2L1 expression in transfected 293T cell line by MAD2L1 monoclonal antibody (M01), clone 2E2-1D6.Lane 1: MAD2L1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged MAD2L1 is approximately 0.03ng/ml as a capture antibody.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex.
Western Blot analysis of MAD2L1 expression in transfected 293T cell line (H00004085-T02) by MAD2L1 MaxPab polyclonal antibody.Lane 1: MAD2L1 transfected lysate(23.50 KDa).Lane 2: Non-transfected lysate.
Western blot using MAD2L1 monoclonal antibody, clone 17D10 ( Cat # MAB1926 ) shows detection of aband at ~24 kDa ( arrowhead ) correspondingto MAD2L1 present in a HeLa whole cell lysate ( lane 1 ).Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE.After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.The membrane was washed and reacted with a 1 : 5,000 dilution of IRDyeTM800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature ( 800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).IRDyeTM800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results.
Western blot using MAD2L1 polyclonal antibody ( Cat # PAB10076 ) shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 ( arrowhead ) present in Jurkat ( lane 1 ) and HeLa ( lane 2 ) whole cell lysates using the 800 nmchannel ( green ).The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide ( lanes 4 and 5 respectively ) which completely blocks antibody staining.Approximately 35 µg of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1200.Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.Molecular weight markers were used for size comparison using the 700 nm channel ( lane 3 ). IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
Human MCF-7; WB Suggested Anti-MAD2L1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; MAD2L1 antibody - middle region (AVARP04002_P050) in Human MCF-7 cells using Western Blot
Western blot analysis of MAD2 expression in Jurkat (Lane 1), HeLa (Lane 2), Raji (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20288AF-N MAD2 antibody.
Immunoprecipitation of MAD2 from Jurkat with AM20288AF-N (Lane 1) or Mouse IgG (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM20288AF-N MAD2 antibody.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Sample (30 µg of whole cell lysate). A: 293T. 12% SDS PAGE. MAD2L1 antibody diluted at 1/1000.
Western blot analysis of MAD2 expression in Raji (A); HepG2 (B); A431 (C) whole cell lysates.
Anti-MAD2L1 mouse monoclonal antibody (TA500617) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAD2L1(RC203273).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1 (RC203273, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAD2L1.
TA305640 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. TA308922 diluted at 1:1000.
Sample (30ug whole cell lysate). A:Hep G2 . B:MOLT4 . C:Raji . 12% SDS PAGE. TA308923 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(TA308923) antibody at 1:500 dilution.
MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAD2L1 antibody [C2C3], C-term (TA308923) dilution: 1:500.
Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(TA308923) antibody at 1:1000 dilution.
Western blot analysis of extracts from A549 cells, using MAD2L1 antibody.The lane on the right is treated with the synthesized peptide.
WB using Anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates(green). Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively). The primary antibody was diluted to 1:1200. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10000.
WB using anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4? with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT.
Predicted band size: 24 kDa . Positive control: Human seminoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm . Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using MAD2L1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using MAD2L1 antibody.
Immunohistochemistry of paraffin-embedded human rectal cancer using MAD2L1 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat testis using MAD2L1 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of U20S cell using MAD2L1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-MAD2L1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303273 | MAD2L1 |
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| Recombinant protein of human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
