MAD4 antibody
Principal name
MAD4 antibody
Alternative names for MAD4 antibody
MAD-4, Max-associated protein 4, MXD4, MAX dimerization protein 4, Max-interacting transcriptional repressor MAD4
Gene ID
10608 (MXD4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of MAD4 antibody
- Kit Component:
- - KN209651G1, MXD4 gRNA vector 1 in pCas-Guide vector
- KN209651G2, MXD4 gRNA vector 2 in pCas-Guide vector
- KN209651D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN.
Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation.
EMBO J. 1995 Nov 15;14(22):5646-59.
PMID: 8521822
Human Jurkat; WB Suggested Anti-MXD4 Antibody. . Titration: 2.5 ug/ml. . Positive Control: Jurkat Whole Cell; MXD4 antibody - N-terminal region (ARP32777_T100) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-MXD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; MXD4 antibody - N-terminal region (ARP32777_P050) in Human Jurkat cells using Western Blot
MXD4 polyclonal antibody ( Cat # PAB6256 ) staining ( 0.5 µg/ml ) of human kidney lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of MXD4 expression in transfected 293T cell line (H00010608-T02) by MXD4 MaxPab polyclonal antibody.Lane 1: MXD4 transfected lysate(22.99 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MXD4 expression in transfected 293T cell line (H00010608-T02) by MXD4 MaxPab polyclonal antibody.Lane 1: MXD4 transfected lysate(22.99 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808862)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808862)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808862)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MXD4 (RC209651, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MXD4.(1:2000)
TA302831 staining (0.5µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAD4 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-MXD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Human Lung Tissue
WB Suggested Anti-MXD4 Antibody; Titration: 2.5 ug/ml; Positive Control: Jurkat Whole Cell
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808862 | MAD4 antibody |
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| MXD4 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
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Mouse | IgG2b | OTI1A2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA302831 | MAD4 (C-term) antibody |
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| Goat Polyclonal Antibody against MXD4 | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA312800 | MAD4 antibody |
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| Rabbit polyclonal anti-MAD4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760343 | MAD4 (full length, N-term HIS tag) |
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| Purified recombinant protein of Homo sapiens MAX dimerization protein 4 (MXD4), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416638 | MXD4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |