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MAdCAM1 antibody

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Principal name

MAdCAM1 antibody

Alternative names for MAdCAM1 antibody

hMAdCAM-1, MAdCAM-1, Mucosal addressin cell adhesion molecule 1

SwissProt ID

O70540 (Rat), Q13477 (Human), Q61826 (Mouse)

Gene ID

8174 (MADCAM1), 17123 (Madcam1), 54266

Ncbi ID

10090, NP_570116.2, NM_130760, NM_130762, NP_570118, NP_570116, NM_013591, BC024372, NM_019317

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Rt (Rat)

Available hosts

Rat, Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAdCAM1 antibody

MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin a;4ß;7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

TA506905 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506906 - MAdCAM1
Anti-MADCAM1 mouse monoclonal antibody (TA506906) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MADCAM1(RC219060).
TA506906 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506916 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506917 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506919 - MAdCAM1
Immunofluorescent staining of HeLa cells using anti-MADCAM1 mouse monoclonal antibody (TA506919).
TA506919 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506920 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506924 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506925 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA506986 - MAdCAM1
Immunofluorescent staining of HeLa cells using anti-MADCAM1 mouse monoclonal antibody (TA506986) at 1:100 dilution.
TA506986 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA507035 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
TA507035 - MAdCAM1
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MADCAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA507088 - MAdCAM1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
UM500105 - MAdCAM1
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
UM500105 - MAdCAM1
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
UM500105 - MAdCAM1
Western blot analysis of  COLO205 cell lysate (35ug) by using anti-MADCAM1 monoclonal antibody.
TP312381 - MAdCAM1
TP319060 - MAdCAM1
TA320419 - MAdCAM1
Staining of mouse TNF alpha stimulated bEnd.3 cell line with Anti-Mouse MAdCAM-1 FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
TA352311 - MAdCAM1
TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE

3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAdCAM1 antibody
MAdCAM1 Mouse IgG1 CA102.2C1 Azide Free Hu E, FN, WB
0.1 mg / €650.00
  OriGene Technologies GmbH

MAdCAM1 antibody
MAdCAM1 Mouse IgG1 314G8 Low Endotoxin Hu E, F, FN, P, WB
0.5 mg / €720.00
  OriGene Technologies GmbH

MAdCAM1 antibody
MAdCAM1 Mouse IgG1 314G8 Purified Hu C, E, F, FN, P, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

3 Item(s)

per page

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Product Type
  1. Primary Antibody (3)
Clonality
  1. Monoclonal (3)
Target
  1. MAdCAM1 (3)
Reactivity
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Presentation
  1. Azide Free (1)
  2. Low Endotoxin (1)
  3. Purified (1)
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  1. Mouse (3)
Manufacturer
  1. OriGene Technologies (3)
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