MAFB antibody
Principal name
MAFB antibody
Alternative names for MAFB antibody
Transcription factor MafB, MAF-B, KRML, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Ncbi ID
9606, NP_005452, NM_005461, NP_005452.2, NM_010658, NM_019316, NP_034788.1
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of MAFB antibody
- Kit Component:
- - KN207311G1, MAFB gRNA vector 1 in pCas-Guide vector
- KN207311G2, MAFB gRNA vector 2 in pCas-Guide vector
- KN207311D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kim, H., et al. Nat. Immunol. 11(8):743-750(2010)
Beaty, T.H., et al. Nat. Genet. 42(6):525-529(2010)
Vatsveen, T.K., et al. Leuk. Res. 33(12):1670-1677(2009)
Zanocco-Marani, T., et al. Exp. Cell Res. 315(11):1798-1808(2009)
Kathiresan, S., et al. Nat. Genet. 41(1):56-65(2009)
Human kidney; MAFB antibody - N-terminal region (ARP33682_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MAFB Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; MAFB antibody - N-terminal region (ARP33682_T100) in Human HepG2 cells using Western Blot
Human Spleen; MAFB antibody - N-terminal region (ARP33683_P050) in Human Spleen cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; MAFB antibody - N-terminal region (ARP33683_P050) in Human HepG2 cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:MAFB. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; MAFB antibody - N-terminal region (ARP58149_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:MAFB. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; MAFB antibody - N-terminal region (ARP58149_P050) in Hum. Fetal Lung cells using Western Blot
Human Muscle; WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; MAFB antibody - N-terminal region (ARP58149_P050) in Human Muscle cells using Western Blot
Human OVCAR-3; WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: OVCAR-3 cell lysate; MAFB antibody - middle region (ARP58150_P050) in Human OVCAR-3 cells using Western Blot
Western blot analysis of MAFB Antibody (C-term) Cat.-No AP52585PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the MAFB antibody detected the MAFB protein (arrow).
Flow cytometric analysis of Jurkat cells using MAFB Antibody (C-term) Cat.-No AP52585PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.
HEK293T cells transfected with either RC207311 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAFB antibody(TA505014), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800279)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800290)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.
HEK293T cells transfected with either RC207311 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAFB antibody(TA800290), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800291)
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-MAFB monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.
HEK293T cells transfected with either RC207311 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAFB antibody(TA800291), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB (RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB.
WB Suggested Anti-MAFB Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate
Human kidney
WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
Human Spleen
WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: OVCAR-3 cell lysate
WB Suggested Anti-MAFB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle
Host:Rabbit; Target Name:MAFB; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:MAFB; Sample Tissue:Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800291 | MAFB antibody |
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| MAFB mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) | |||||||
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Mouse | IgG1 | OTI2A6 | Purified | Hu | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +13 additional images |
Top Selling product! Highly validated antibody!
|
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| TA800290 | MAFB antibody |
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| MAFB mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) | |||||||
 |
Mouse | IgG2b | OTI1E9 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
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| TA505014 | MAFB antibody |
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| MAFB mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) | |||||||
 |
Mouse | IgG1 | OTI3F6 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA800279 | MAFB antibody |
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| MAFB mouse monoclonal antibody, clone OTI1F1 (formerly 1F1) | |||||||
 |
Mouse | IgG1 | OTI1F1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA800349 | MAFB antibody |
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| MAFB mouse monoclonal antibody, clone OTI2F12 (formerly 2F12) | |||||||
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Mouse | IgG1 | OTI2F12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP52585PU-N | MAFB (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY401676 | MAFB overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204681L1V | Lenti ORF particles, Mafb (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), 200 uL, >10^7 TU/mL |
|
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208507L2V | Lenti ORF particles, Mafb (GFP-tagged ORF) - Rat v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) (Mafb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208507L1V | Lenti ORF particles, Mafb (Myc-DDK-tagged ORF) - Rat v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) (Mafb), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||