MAGE-4 antibody
Principal name
MAGE-4 antibody
Alternative names for MAGE-4 antibody
MAGEA4, MAGE4, MAGE 4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4
SwissProt ID
P43358 (Human)
Gene ID
4103 (MAGEA4)
Ncbi ID
NP_001011548.1, NP_001011548, 9606, AAH17723, NP_001011549, NP_001011550, NM_001011549, NM_001011548, NM_002362, NM_001011550
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of MAGE-4 antibody
MAGEA4 is a member of the MAGEA family. The members of this family are proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.
General readings
Fradet Y., et al. (2009) Int J Cancer. 125(6):1365-71.
Nishimura T., et al. (2009) Br J Cancer. 100(7):1135-43.
Immunofluorescence of monoclonal antibody to MAGEA4 on HeLa cell. [antibody concentration 10 ug/ml]
MAGEA4 monoclonal antibody (M01), clone 3D12 Western Blot analysis of MAGEA4 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged MAGEA4 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of MAGEA4 expression in transfected 293T cell line by MAGEA4 polyclonal antibody (A01).Lane1:MAGEA4 transfected lysate(34.9 KDa).Lane2:Non-transfected lysate.
MAGEA4 polyclonal antibody (A01), Lot # 060814QCS1. Western Blot analysis of MAGEA4 expression in HeLa.
Western Blot analysis of MAGEA4 expression in transfected 293T cell line (H00004103-T01) by MAGEA4 MaxPab polyclonal antibody.Lane 1: MAGEA4 transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.
Figure 1. Staining MAGEA4 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 polyclonal antibody ( Cat # PAB4746 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
The MAGEA4 polyclonal antibody ( Cat # PAB4746 ) is used in Western blot to detect MAGEA4 in A-375 cell lysate.
MAGEA4 MaxPab rabbit polyclonal antibody. Western Blot analysis of MAGEA4 expression in mouse spleen.
Western Blot analysis of MAGEA4 expression in transfected 293T cell line (H00004103-T01) by MAGEA4 MaxPab polyclonal antibody.Lane 1: MAGEA4 transfected lysate(34.90 KDa).Lane 2: Non-transfected lysate.
Hum. Fetal Heart; Host: Rabbit . Target Name: MAGEA4 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; MAGEA4 antibody - C-terminal region (ARP54410_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Liver; Host: Rabbit . Target Name: MAGEA4 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; MAGEA4 antibody - C-terminal region (ARP54410_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: MAGEA4 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; MAGEA4 antibody - C-terminal region (ARP54410_P050) in Hum. Fetal Lung cells using Western Blot
Human HepG2; WB Suggested Anti-MAGEA4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; MAGEA4 antibody - C-terminal region (ARP54410_P050) in Human HepG2 cells using Western Blot
TOP: Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. BOTTOM: Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-MAGE-A4 Pab is used in Western blot to detect MAGE-A4 in A375 cell lysate.
Western Blot analysis of MAGEA4 expression in transfected 293T cell line (H00004103-T01) by MAGEA4 MaxPab polyclonal antibody.Lane 1: MAGEA4 transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.
Recombinant human MAGEA4, 1-317aa, His-tagged
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505361)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505361)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505361)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505361)
Anti-MAGEA4 mouse monoclonal antibody (TA505361) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAGEA4(RC223991).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (RC223991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505362)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (RC223991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (RC223991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4.
Predicted cell location: Cytoplasm . Positive control: Human tonsil and stomach cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil and stomach cancer tissue using MAGEA4 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil and stomach cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil and stomach cancer tissue using MAGEA4 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using MAGEA4 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x100)
WB Suggested Anti-MAGEA4 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: MAGEA4; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: MAGEA4; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: MAGEA4; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323460 | MAGE-4 antibody |
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| Anti-MAGEA4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323461 | MAGE-4 antibody |
||||||
| Anti-MAGEA4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349177 | MAGE-4 antibody |
||||||
| Rabbit Polyclonal Anti-MAGEA4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP13131PU-N | MAGE-4 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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