MAGE-B4 antibody
Principal name
MAGE-B4 antibody
Alternative names for MAGE-B4 antibody
MAGEB4, Melanoma-associated antigen B4
SwissProt ID
O15481 (Human)
Gene ID
4115 (MAGEB4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MAGE-B4 antibody
- Kit Component:
- - KN206060G1, MAGEB4 gRNA vector 1 in pCas-Guide vector
- KN206060G2, MAGEB4 gRNA vector 2 in pCas-Guide vector
- KN206060D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Anti-MAGEB4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Predicted band size: 39 kDa . Positive control: Human brain glioma tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using MAGEB4 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200)
WB Suggested Anti-MAGEB4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysateMAGEB4 is supported by BioGPS gene expression data to be expressed in HepG2
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323020 | MAGE-B4 antibody |
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| Anti-MAGEB4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306060 | MAGE-B4 |
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| Recombinant protein of human melanoma antigen family B, 4 (MAGEB4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419379 | MAGEB4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |