MAGE-D1 antibody
Principal name
MAGE-D1 antibody
Alternative names for MAGE-D1 antibody
Melanoma-associated antigen D1, MAGED1, NRAGE, Neurotrophin receptor-interacting MAGE homolog, MAGE tumor antigen CCF
Ncbi ID
9606, NP_001005332.1, NP_062765, NP_001005333, AAH32473, NM_006986, NM_001005332, NM_001005333, NP_062765.1, NM_053409
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of MAGE-D1 antibody
- Kit Component:
- - KN309667G1, Maged1 gRNA vector 1 in pCas-Guide vector
- KN309667G2, Maged1 gRNA vector 2 in pCas-Guide vector
- KN309667D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dombret C. et al. (2012) Hum Mol Genet. 21:4703-4717
Mouri ,. et al. (2012) J Neurosci. 32:4562-4580.
MAGED1 polyclonal antibody (A01), Lot # 060927JCS1 Western Blot analysis of MAGED1 expression in 293 ( Cat # L026V1 ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAGED1 polyclonal antibody ( Cat # PAB4756 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
The MAGED1 polyclonal antibody ( Cat # PAB4756 ) is used in Western blot to detect MAGED1 in A-549 cell lysate.
Immunoperoxidase of monoclonal antibody to MAGED1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
MAGED1 monoclonal antibody (M06), clone 1E1. Western Blot analysis of MAGED1 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged MAGED1 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of MAGED1 expression in transfected 293T cell line (H00009500-T01) by MAGED1 MaxPab polyclonal antibody.Lane 1: MAGED1 transfected lysate(85.58 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-MAGED1 Ctr Antibody is used in Western blot to detect MAGED1 in A549 lysate.
MAGED1 MaxPab rabbit polyclonal antibody. Western Blot analysis of MAGED1 expression in mouse spleen.
Western Blot analysis of MAGED1 expression in transfected 293T cell line (H00009500-T01) by MAGED1 MaxPab polyclonal antibody.Lane 1: MAGED1 transfected lysate(86.20 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody AP26048PU-N and the same antibody preincubated with blocking peptide.
Predicted band size: 86 kDa . Positive control: A549, Jurkat and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Jurkat cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/1200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer using MAGED1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x 200)
Predicted band size: 86 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer using MAGED1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x 200)
Western blot analysis of A549 cell lysate using MAGED1 antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323493 | MAGE-D1 antibody |
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| Anti-MAGED1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323494 | MAGE-D1 antibody |
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| Anti-MAGED1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA326921 | MAGE-D1 antibody |
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| MAGED1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP13141PU-N | MAGE-D1 antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP26048PU-N | MAGE-D1 (C-term) antibody |
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 |
Rabbit | Aff - Purified | Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP26048PU-S | MAGE-D1 (C-term) antibody |
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|
Rabbit | Aff - Purified | Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416269 | MAGED1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY423858 | MAGED1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY423859 | MAGED1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214811L2V | Lenti ORF particles, Maged1 (GFP-tagged ORF) - Rat melanoma antigen, family D, 1 (Maged1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,570.00
|
|
| OriGene Technologies, Inc. | ||
| RR214811L1V | Lenti ORF particles, Maged1 (Myc-DDK-tagged ORF) - Rat melanoma antigen, family D, 1 (Maged1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||