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MAP-2 antibody

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Principal name

MAP-2 antibody

Alternative names for MAP-2 antibody

Microtubule-associated protein 2, MAP2, Neuronal Marker

SwissProt ID

P11137 (Human), P15146 (Rat), P20357 (Mouse), Q28869 (Bovin)

Gene ID

4133 (MAP2)

Ncbi ID

NP_002365, 9606, NP_002365.3, 10116, NP_114033, NM_031847

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Chk (Chicken), Quail, Xen (Xenopus), Prim (Primate), Av (Birds), Fe (Feline), Por (Porcine), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of MAP-2 antibody

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alterte transcription, and all share a C-termil sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neurol cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neurol cells, their perikarya and neurol dendrites. In contrast tau is found predomintly in neurol axons.

General readings

1. Fontaine-Lenoir, V.; Chambraud, B.; Fellous, A.; David, S.; Duchossoy, Y.; Baulieu, E.-E.; Robel, P. :
Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. Proc. Nat. Acad. Sci. 103: 4711-4716, 2006.
2. Neve, R. L.; Harris, P.; Kosik, K. S.; Kurnit, D. M.; Donlon, T. A. : Identification of cDNA clones for the human microtubule-associated protein tau and chromosomal localization of the genes for tau and microtubule-associated protein 2. Molec. Brain Res. 1: 271-280, 1986.
3. Kalcheva, N.; Albala, J.; O'Guin, K.; Rubino, H.; Garner, C.; Shafit-Zagardo, B. : Genomic structure of human microtubule-associated protein 2 (MAP-2) and characterization of additional MAP-2 isoforms. Proc. Nat. Acad. Sci. 92: 10894-10898, 1995.

AM20513PU-N - MAP-2
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with MAP2 antibody AM20513PU-N followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM20513PU-N - MAP-2
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with MAP2 antibody AM20513PU-N followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM20513PU-N - MAP-2
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with MAP2 antibody AM20513PU-N followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM20638PU-N - MAP-2
Paraffin-Embedded Section of Rat Brain Tissue stained with Monoclonal Anti-MAP2 antibody
AP11882PU-N - MAP-2
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAP2 Antibody (C-term) (AP11882PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11882PU-N - MAP-2
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11882PU-N - MAP-2
Western blot analysis of MAP2 (arrow) using MAP2 Antibody (C-term) (AP11882PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2 (Lane 2) (Origene Technologies).
AP20610PU-N - MAP-2
Immunohistochemistry (IHC) analyzes of MAP-2 antibody (Cat.-No.: AP20610PU-N) in paraffin-embedded human brain tissue.
AP20610PU-N - MAP-2
Western blot (WB) analysis of MAP-2 antibody (Cat.-No.: AP20610PU-N) in extracts from mouse brain cells.
AP31808PU-N - MAP-2
Dissociated cell cultures of an e13 Mouse brain showing MAP-2 (green staining) of neurons. DAPI (blue staining) allows visualization of nuclei.
BM2472 - MAP-2
Formalin-fixed, paraffin-embedded human brain stained with Microtubule-Associated Protein 2a,b Ab (Cat. BM2472) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons and dendrites.
H00004133-B01 - MAP-2
Western Blot analysis of MAP2 expression in transfected 293T cell line (H00004133-T01) by MAP2 MaxPab polyclonal antibody.Lane 1: MAP2 transfected lysate(51.81 KDa).Lane 2: Non-transfected lysate.
H00004133-D01P - MAP-2
Western Blot analysis of MAP2 expression in transfected 293T cell line (H00004133-T01) by MAP2 MaxPab polyclonal antibody.Lane 1: MAP2 transfected lysate(49.60 KDa).Lane 2: Non-transfected lysate.
MAB3866 - MAP-2
Western Blotting analysis ( reducing conditions ) of microtubules partially purified from porcine brain lysate.Lane 1 : Immunostaining with MAP2 monoclonal antibody, clone MT-01 ( Cat # MAB3866 ).Lane 2 : Immunostaining with MAP2 monoclonal antibody, clone MT-07 ( Cat # MAB3636 ).Lane 3 : Immunostaining with MAP2 monoclonal antibody, clone MT-08 ( Cat # MAB3637 ).
PAB3854 - MAP-2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP2 polyclonal antibody ( Cat # PAB3854 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SM3121P - MAP-2
Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01). Lane 2: immunostaining with anti-MAP2ab (MT-07; SM3159P). Lane 3: immunostaining with anti-MAP2ab (MT-08; SM3160P).
SM3159P - MAP-2
Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate.Lane 1: immunostaining with anti-MAP2ab (MT-01;SM3121P). Lane 2: immunostaining with anti-MAP2ab (MT-07; SM3159P). Lane 3: immunostaining with anti-MAP2ab (MT-08; SM3160P).
SM3160P - MAP-2
Western Blotting analysis (reducing conditions) of microtubules partially purified from Porcine brain lysate. Lane 1: Immunostaining with anti-MAP2ab (MT-01, SM3121P). Lane 2: Immunostaining with anti-MAP2ab (MT-07, SM3159P). Lane 3: Immunostaining with anti-MAP2ab (MT-08, SM3160P).
TP316775 - MAP-2
TP321637 - MAP-2
TA309162 - MAP-2
Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein.
TA309740 - MAP-2
Western Blot: MAP2 Antibody (5H11) - Western blot of whole rat brain lysate probed with anti-MAP2 antibody to MAP2. Note that the strong single band running at about 280 kDa corresponds to MAP2.
TA324336 - MAP-2
MAP2 Antibody (Cat. #TA324336) western blot analysis in MCF-7 cell line lysates (15ug/lane).This demonstrates the MAP2 antibody detected the MAP2 protein (arrow).(1:1000)
TA324336 - MAP-2
MAP2 Antibody (Ascites) (Cat. #TA324336)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAP2 Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
TA324336 - MAP-2
Confocal immunofluorescent analysis of MAP2 Antibody (Cat#TA324336) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
TA347000 - MAP-2
Western blot detection of MAP2(N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.
TA347000 - MAP-2
Immunocytochemistry of HeLa cells using anti-MAP2 (N-terminus) mouse mAb diluted 1:150.
TA347000 - MAP-2
Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.
TA347836 - MAP-2
Western blot analysis of extract from C6 cells using MAP2 Antibod
TA347897 - MAP-2
Western blot analysis of MAP2 expression in mouse brain
TA323368 - MAP-2
Predicted band size: 200 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds)
TA323368 - MAP-2
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using Map2 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323369 - MAP-2
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using Map2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA323369 - MAP-2
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using Map2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA327383 - MAP-2
Western blot analysis of extracts of various cell lines, using MAP2 antibody.
TA336617 - MAP-2
Western Blot: MAP2 Antibody [TA336617] - Western blot analysis of MAP2 expression in bovine brain extract using TA336617.
TA336617 - MAP-2
Immunocytochemistry/Immunofluorescence: MAP2 Antibody [TA336617] - Mixed neuron/glial cultures stained with TA336617 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neuron
TA337109 - MAP-2
Western Blot: MAP2 Antibody (4H5) [TA337109] - Western blot of rat brain extract; note that the strong single band running at about 280kDa corresponds to MAP2.
TA337109 - MAP-2
Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [TA337109] - Mixed neuron and glia cultures stained with TA337109 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). TA337109 reveals strong cytoplasmic staining for of dendrite
TA352265 - MAP-2
Staining of MAP2, Clone SMI 52, at 1/6000 on formalin-fixed paraffin-embedded mouse brain.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP-2 antibody
of primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue. Chicken Serum Hu, Ms, Prim, Rt C, ICC/IF, WB
50 µl / €390.00
  Neuromics Antibodies
+1 additional image

Top Selling product!

MAP-2 antibody
  Chicken Anti-Microtubule Associated Protein 2 (MAP2) Antibody  
Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Chicken Ig Ig Fraction Bov, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €355.00
  OriGene Technologies, Inc.

MAP-2 antibody
  Anti-Map2 Rabbit Polyclonal Antibody  
Predicted band size: 200 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MAP-2 antibody
  Anti-Map2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using Map2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MAP-2 antibody
  MAP2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MAP2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MAP-2 antibody
  Chicken Polyclonal MAP2 Antibody  
Western Blot: MAP2 Antibody [TA336617] - Western blot analysis of MAP2 expression in bovine brain extract using TA336617. Chicken IgY Purified Bov, Hu, Ms, Rt C, ICC/IF, WB
50 µl / €395.00
  OriGene Technologies, Inc.
+1 additional image

MAP-2 antibody
  Rabbit Polyclonal Anti-MAP2 Antibody  
Western blot analysis of extract from C6 cells using MAP2 Antibod Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MAP-2 antibody
  Rabbit Polyclonal Anti-MAP 2 Antibody  
Western blot analysis of MAP2 expression in mouse brain Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MAP-2 (C-term) antibody
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAP2 Antibody (C-term) (AP11882PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

MAP-2 antibody
Immunohistochemistry (IHC) analyzes of MAP-2 antibody (Cat.-No.: AP20610PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

MAP-2 antibody
Dissociated cell cultures of an e13 Mouse brain showing MAP-2 (green staining) of neurons. DAPI (blue staining) allows visualization of nuclei. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.2 mg / €550.00
  OriGene Technologies GmbH

MAP-2 antibody
MAP2 positive cultured neurons (green) from an e13.5 mouse brain. Neuclei are in blue. Chicken Aff - Purified Hu, Ms C, ICC/IF
0.1 ml / €180.00
  Neuromics Antibodies

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