MAP2K7 antibody
Principal name
MAP2K7 antibody
Alternative names for MAP2K7 antibody
MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7, NKK2, MEK7, MKK7, MAP kinase kinase 7, Dual specificity mitogen-activated protein kinase kinase 7
Ncbi ID
NP_660186.1, 9606, NP_660186, NM_145185, NP_001036022.1, NM_001164172, NM_011944, NM_001042557, NM_001025425
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of MAP2K7 antibody
MAP2K7(Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.
Detection of endogenous MKK7: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK7-10F7 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
MAP2K7 monoclonal antibody (M01), clone 4E8 Western Blot analysis of MAP2K7 expression in Hela NE ( Cat # L013V3 ).
MAP2K7 monoclonal antibody (M02), clone 4D2. Western Blot analysis of MAP2K7 expression in Raw 264.7 ( Cat # L024V1 ).
MAP2K7 monoclonal antibody (M02), clone 4D2 Western Blot analysis of MAP2K7 expression in Hela NE ( Cat # L013V3 ).
MAP2K7 monoclonal antibody (M02), clone 4D2. Western Blot analysis of MAP2K7 expression in PC-12 ( Cat # L012V1 ).
Immunoperoxidase of monoclonal antibody to MAP2K7 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.2 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK8 and MAP2K7 HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-MAP2K7 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of MAP2K7 expression in transfected 293T cell line by MAP2K7 monoclonal antibody (M04), clone 2G5.Lane 1: MAP2K7 transfected lysate(47.485 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MAP2K7 expression in transfected 293T cell line by MAP2K7 monoclonal antibody (M05), clone 6A4.Lane 1: MAP2K7 transfected lysate(47.485 KDa).Lane 2: Non-transfected lysate.
MAP2K7 polyclonal antibody (A01), Lot # 061025JCS1. Western Blot analysis of MAP2K7 expression in NIH/3T3.
MAP2K7 polyclonal antibody (A01), Lot # 061025JCS1. Western Blot analysis of MAP2K7 expression in Raw 264.7.
MAP2K7 polyclonal antibody (A01), Lot # 061025JCS1 Western Blot analysis of MAP2K7 expression in Hela NE ( Cat # L013V3 ).
MAP2K7 polyclonal antibody (A01), Lot # 061025JCS1. Western Blot analysis of MAP2K7 expression in PC-12.
Western Blot analysis of MAP2K7 expression in transfected 293T cell line by MAP2K7 monoclonal antibody (M07), clone 2C12.Lane 1: MAP2K7 transfected lysate(47.485 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MAP2K7 expression in transfected 293T cell line (H00005609-T01) by MAP2K7 MaxPab polyclonal antibody.Lane 1: MAP2K7 transfected lysate(46.86 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MAP2K7 expression in transfected 293T cell line (H00005609-T02) by MAP2K7 MaxPab polyclonal antibody.Lane 1: MAP2K7 transfected lysate(48.20 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-MAP2K7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; MAP2K7 antibody - middle region (ARP49025_P050) in Human Jurkat cells using Western Blot
Western blot (WB) analysis of MEK-7 antibody (Cat.-No.: AP21117PU-N) in extracts from HeLa cells.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody AP55764PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody AP55764PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody AP55765PU-N. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of extracts of various celllines, using MAP2K7 antibody.
Immunohistochemistry of paraffin-embedded mouse kidney using MAP2K7 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K7 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat spleen using MAP2K7 antibody at dilution of 1:100 (400x lens).
Immunofluorescence analysis of U2OS cell using MAP2K7 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from 3T3 cells treated with insulin (0.01U/ml, 15mins) and 3T3 cells treated with EGF (200ng/ml, 30mins), using MAP2K7 (Phospho-Ser271) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody.The picture on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327324 | MAP2K7 antibody |
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| MAP2K7 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AM06701SU-N | MAP2K7 antibody |
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 |
Mouse | IgG1 | 4E5 | Ascites | Hu | E, F, ICC/IF, P, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
| +5 additional images |
Highly validated antibody! |
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| TA312581 | MAP2K7 antibody |
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| Rabbit polyclonal MAP2K7 (Ser271) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312582 | MAP2K7 antibody |
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| Rabbit polyclonal MAP2K7 (Thr275) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP21117PU-N | MAP2K7 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP55764PU-N | MAP2K7 pSer271 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP55764PU-S | MAP2K7 pSer271 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP55765PU-N | MAP2K7 pThr275 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55765PU-S | MAP2K7 pThr275 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN309725 | Map2k7 - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309725BN | Map2k7 - mouse gene knockout kit via CRISPR |
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|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309725LP | Map2k7 - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309725RB | Map2k7 - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313868 | MAP2K7 |
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| Recombinant protein of human mitogen-activated protein kinase kinase 7 (MAP2K7) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408043 | MAP2K7 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
