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MAP4K1 antibody

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Principal name

MAP4K1 antibody

Alternative names for MAP4K1 antibody

MEKKK 1, MAPK/ERK kinase kinase kinase 1, HPK1, Hematopoietic progenitor kinase, Mitogen-activated protein kinase kinase kinase kinase 1

SwissProt ID

P70218 (Mouse), Q92918 (Human)

Gene ID

11184 (MAP4K1), 26411 (Map4k1)

Ncbi ID

9606, NP_001036065.1, NP_009112, NP_001036065, NM_007181, NP_032305.2, BC005433, NM_008279, NM_001042600

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Por (Porcine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot)

Background of MAP4K1 antibody

The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

General readings

1. Hu M.C.-T., Genes Dev. 10:2251-2264(1996).
2. Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
3,. Wissing J., Mol. Cell. Proteomics 6:537-547(2007).

AP06749PU-N - MAP4K1
Western blot (WB) analysis of HPK1 antibody (Cat.-No.: AP06749PU-N) in extracts from HepG2 cells.
PAB3188 - MAP4K1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K1 polyclonal antibody ( Cat # PAB3188 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3188 - MAP4K1
Western blot analysis of MAP4K1 polyclonal antibody ( Cat # PAB3188 ) in A-375 cell lysate. MAP4K1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PAB6178 - MAP4K1
MAP4K1 polyclonal antibody ( Cat # PAB6178 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
MAB0813 - MAP4K1
Immunoblot analysis of cell lysates. Lane 1 : Jurkat T cell lysate. Lane 2 : Ramos cell lysate.
AP12868PU-N - MAP4K1
Dot blot analysis of anti-MAP4K1-pThr165 Phospho-specific Pab (Cat#AP12868PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
AP12870PU-N - MAP4K1
Dot blot analysis of anti-MAP4K1-pSer171 Phospho-specific Pab (Cat#AP12870PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
AP14874PU-N - MAP4K1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14874PU-N - MAP4K1
Western blot analysis of anti-HPK1 Pab in A375 cell lysate. HPK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PAB8123 - MAP4K1
Dot blot analysis of MAP4K1 (phospho T165) polyclonal antibody ( Cat # PAB8123 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
PAB8125 - MAP4K1
Dot blot analysis of MAP4K1 (phospho S171) polyclonal antibody ( Cat # PAB8125 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
ARP46044_P050 - MAP4K1
Human 721_B; WB Suggested Anti-MAP4K1 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. MAP4K1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; MAP4K1 antibody - N-terminal region (ARP46044_P050) in Human 721_B cells using Western Blot
C11444-1 - MAP4K1
Western blot analysis of extracts from HepG2 cells, using MEKKK 1 Antibody. The lane on the right is treated with the synthesized peptide.
AM31910SU-N - MAP4K1
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA302799 - MAP4K1
TA302799 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA322439 - MAP4K1
Predicted cell location: Cytoplasm. Positive control: Human brian and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brian cancer tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322439 - MAP4K1
Predicted cell location: Cytoplasm. Positive control: Human brian and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA322440 - MAP4K1
Predicted cell location: Cytoplasm. Positive control: Human brain and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322440 - MAP4K1
Predicted cell location: Cytoplasm. Positive control: Human brain and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA326238 - MAP4K1
Western blot analysis of extracts from HepG2 cells, using MEKKK 1 antibody.The lane on the right is treated with the synthesized peptide.
TA338986 - MAP4K1
WB Suggested Anti-MAP4K1 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate.MAP4K1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP4K1 (N-term) antibody
  Goat Polyclonal Antibody against MAP4K1  
TA302799 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

MAP4K1 antibody
  Anti-MAP4K1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human brian and colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brian cancer tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MAP4K1 antibody
  Anti-MAP4K1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human brain and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using MAP4K1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MAP4K1 antibody
  Rabbit polyclonal MEKKK 1 antibody  
Western blot analysis of extracts from HepG2 cells, using MEKKK 1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

MAP4K1 antibody
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2A1 Purified Hu IP, P, WB
50 µl / €425.00
  OriGene Technologies GmbH

MAP4K1 antibody
Western blot (WB) analysis of HPK1 antibody (Cat.-No.: AP06749PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €370.00
  OriGene Technologies GmbH

MAP4K1 pThr165 antibody
Dot blot analysis of anti-MAP4K1-pThr165 Phospho-specific Pab (Cat#AP12868PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €415.00
  OriGene Technologies GmbH

MAP4K1 pSer171 antibody
Dot blot analysis of anti-MAP4K1-pSer171 Phospho-specific Pab (Cat#AP12870PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €415.00
  OriGene Technologies GmbH

MAP4K1 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

Map4k1 - mouse gene knockout kit via CRISPR
Map4k1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Map4k1 - mouse gene knockout kit via CRISPR
Map4k1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Map4k1 - mouse gene knockout kit via CRISPR
Map4k1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Map4k1 - mouse gene knockout kit via CRISPR
Map4k1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MAP4K1 overexpression lysate
MAP4K1 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Map4k1 (GFP-tagged) - Mouse mitogen activated protein kinase kinase kinase kinase 1 (cDNA clone MGC:5927 IMAGE:3494470), 200 uL, >10^7 TU/mL
Lenti ORF particles, Map4k1 (GFP-tagged) - Mouse mitogen activated protein kinase kinase kinase kinase 1 (cDNA clone MGC:5927 IMAGE:3494470), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Map4k1 (GFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Map4k1 (GFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), 200 uL, >10^7 TU/mL  
200 µl / €1,640.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Map4k1 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase kinase kinase 1 (cDNA clone MGC:5927 IMAGE:3494470), 200 uL, >10^7 TU/mL
Lenti ORF particles, Map4k1 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase kinase kinase 1 (cDNA clone MGC:5927 IMAGE:3494470), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Map4k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Map4k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), 200 uL, >10^7 TU/mL  
200 µl / €1,470.00
  OriGene Technologies, Inc.
 

18 Item(s)

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18 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (4)
  4. Primary Antibody (9)
Clonality
  1. Polyclonal (8)
  2. Monoclonal (1)
Target
  1. MAP4K1 (9)
Property
  1. C-term (1)
  2. N-term (1)
  3. pSer171 (1)
  4. pThr165 (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (3)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
  4. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (5)
  2. Aff - Purified (4)
Host
  1. Rabbit (7)
  2. Goat (1)
  3. Mouse (1)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Dot blot (Dot) (2)
  3. IHC (2)
  4. Paraffin Sections (P) (2)
Species (CRISPR)
  1. Mouse (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (18)
Service
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