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MAPKAP Kinase-2 antibody

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Principal name

MAPKAP Kinase-2 antibody

Alternative names for MAPKAP Kinase-2 antibody

MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MK2, MAPKAPK2, MAP kinase-activated protein kinase 2

SwissProt ID

P49137 (Human), P49138 (Mouse), P49139 (Rabit)

Gene ID

9261 (MAPKAPK2), 17164 (Mapkapk2)

Ncbi ID

NP_004750.1, 9606, 5874, 10090, NP_004750, NM_004635, NM_004759, NM_032960, NP_116584, 9986

Available reactivities

Bov (Bovine), Can (Canine), Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Rb (Rabbit), Xen (Xenopus), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection))

Background of MAPKAP Kinase-2 antibody

MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.

H00009261-M01 - MAPKAP Kinase-2
Immunoperoxidase of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
H00009261-M01 - MAPKAP Kinase-2
Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and MAPKAPK2 HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MAPKAPK2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00009261-M01 - MAPKAP Kinase-2
Immunofluorescence of monoclonal antibody to MAPKAPK2 on HeLa cell. [antibody concentration 10 ug/ml]
H00009261-M01 - MAPKAP Kinase-2
MAPKAPK2 monoclonal antibody (M01), clone 2B3 Western Blot analysis of MAPKAPK2 expression in K-562 ( Cat # L009V1 ).
H00009261-M01 - MAPKAP Kinase-2
Detection limit for recombinant GST tagged MAPKAPK2 is approximately 0.1ng/ml as a capture antibody.
H00009261-M10 - MAPKAP Kinase-2
MAPKAPK2 monoclonal antibody (M10), clone 3A7 Western Blot analysis of MAPKAPK2 expression in PC-12 ( Cat # L012V1 ).
H00009261-M08 - MAPKAP Kinase-2
Immunoperoxidase of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
H00009261-M08 - MAPKAP Kinase-2
MAPKAPK2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of MAPKAPK2 expression in NIH/3T3(Cat # L018V1 ).
H00009261-M08 - MAPKAP Kinase-2
MAPKAPK2 monoclonal antibody (M08), clone 3B8 Western Blot analysis of MAPKAPK2 expression in Jurkat ( Cat # L017V1 ).
H00009261-M08 - MAPKAP Kinase-2
MAPKAPK2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of MAPKAPK2 expression in PC-12(Cat # L012V1 ).
AP02538PU-S - MAPKAP Kinase-2
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK-2 pThr334 antibody AP02538PU
AP02538PU-S - MAPKAP Kinase-2
Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using MAPKAPK-2 pThr334 antibody AP02538PU (Red).
AP02538PU-S - MAPKAP Kinase-2
Figure 2. Western blot analysis of extract from HeLa cells treated with UV (20min), using MAPKAPK-2 antibody AP02791PU (Lane 1 and 2) and MAPKAPK-2 pThr334 antibody AP02538PU (Lane 3 and 4).
AP02791PU-S - MAPKAP Kinase-2
Immunofluorescence staining of methanol-fixed HeLa cells using MAPKAPK-2 antibody (Red).
AP02791PU-S - MAPKAP Kinase-2
Western Blot analysis of extracts from 293 cells using MAPKAPK-2 antibody and the same antibody preincubated with blocking peptide.
AP06619PU-N - MAPKAP Kinase-2
Immunohistochemistry (IHC)analyzesof MAPKAPK-2 antibody (Cat.-No.: AP06619PU-N) in paraffin-embedded human breast carcinoma tissue.
AP06619PU-N - MAPKAP Kinase-2
Western blot (WB) analysis of MAPKAPK-2 antibody (Cat.-No.: AP06619PU-N) in extracts from raw264.7 cells.
AP12635PU-N - MAPKAP Kinase-2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP12635PU-N - MAPKAP Kinase-2
Dot blot analysis of anti-Phospho-MAPKAPK2-S272 Antibody (Cat.#AP12635PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
H00009261-D01P - MAPKAP Kinase-2
Western Blot analysis of MAPKAPK2 expression in transfected 293T cell line (H00009261-T04) by MAPKAPK2 MaxPab polyclonal antibody.Lane 1: MAPKAPK2 transfected lysate(45.60 KDa).Lane 2: Non-transfected lysate.
AP21090PU-N - MAPKAP Kinase-2
Immunohistochemistry (IHC) analyzes of MAPKAPK-2 antibody (Cat.-No.: AP21090PU-N) in paraffin-embedded human heart tissue.
AP21090PU-N - MAPKAP Kinase-2
Western blot (WB) analysis of MAPKAPK-2 antibody (Cat.-No.: AP21090PU-N) in extracts from HUVEC cells.
AP21135PU-N - MAPKAP Kinase-2
Immunohistochemistry (IHC) analyzes of MAPKAPK-2 antibody (Cat.-No.: AP21135PU-N) in paraffin-embedded human breast carcinoma tissue.
AP21135PU-N - MAPKAP Kinase-2
Western blot (WB) analysis of MAPKAPK-2 antibody (Cat.-No.: AP21135PU-N) in extracts from COS cells.
A0018-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is treated with the synthesized peptide.
A0018-1 - MAPKAP Kinase-2
Western blot analysis of extracts from NIH-3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is treated with the synthesized peptide.
A0677-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human heart, using MAPKAPK-2 (Phospho-Thr222) Antibody. The picture on the right is treated with the synthesized peptide.
A0677-1 - MAPKAP Kinase-2
Western blot analysis of extracts from COS7 cells treated with UV 15', using MAPKAPK-2 (Phospho-Thr222) Antibody. The lane on the right is treated with the synthesized peptide.
A8363-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Ser272) Antibody. The picture on the right is treated with the synthesized peptide.
A8363-1 - MAPKAP Kinase-2
Western blot analysis of extracts from RAW264.7 cells treated with UV 15', using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is treated with the synthesized peptide.
B0018-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK2 (Ab-334) Antibody. The picture on the right is treated with the synthesized peptide.
B0018-1 - MAPKAP Kinase-2
Western blot analysis of extracts from HeLa cells, treated with Ca2+ 40nM 30', using MAPKAPK2 (Ab-334) Antibody. The lane on the right is treated with the synthesized peptide.
B0677-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MAPKAPK2 (Ab-222) Antibody. The picture on the right is treated with the synthesized peptide.
B0677-1 - MAPKAP Kinase-2
Western blot analysis of extracts from HuvEc cells, treated with Adriamycin 0.5uM 3h, using MAPKAPK2 (Ab-222) Antibody. The lane on the right is treated with the synthesized peptide.
B8363-1 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK2 (Ab-272) Antibody. The picture on the right is treated with the synthesized peptide.
B8363-1 - MAPKAP Kinase-2
Western blot analysis of extracts from COS cells, using MAPKAPK2 (Ab-272) Antibody. The lane on the right is treated with the synthesized peptide.
ARP60320_P050 - MAPKAP Kinase-2
Human HepG2; WB Suggested Anti-MAPKAPK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; MAPKAPK2 antibody - middle region (ARP60320_P050) in Human HepG2 cells using Western Blot
AP32810PU-N - MAPKAP Kinase-2
AP32810PU-N  (0.5 µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
AP32810PU-N - MAPKAP Kinase-2
HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PU-N (0.5 µg/ml) in the left panel and with AP32811PU-N (0.5 µg/ml) in the right panel.
AP32810PU-N - MAPKAP Kinase-2
AP32810PU-N and AP32811PU-N (1.5 µg) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.
AP32811PU-N - MAPKAP Kinase-2
HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PUN (0.5 μg/ml) in the left panel and with AP32811PU-N (0.5 μg/ml) in the right panel.
AP32811PU-N - MAPKAP Kinase-2
AP32810PU-N and AP32811PU-N (1.5 μg) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with Rabbit anti-MK2.
AP55823PU-S - MAPKAP Kinase-2
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody AP55823PU-N (left)or the same antibody preincubated with blocking peptide (right).
AP55823PU-S - MAPKAP Kinase-2
Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody AP55823PU-N.The lane on the right is treated with the antigen-specific peptide.
TP308563 - MAPKAP Kinase-2
TP320487 - MAPKAP Kinase-2
TA312933 - MAPKAP Kinase-2
Western blot analysis of extracts from COS cells, using MAPKAPK2 (Ab-272) antibody.The lane on the right is treated with the synthesized peptide.
TA312933 - MAPKAP Kinase-2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Ab-272) antibody.The picture on the right is treated with the synthesized peptide.
TA319252 - MAPKAP Kinase-2
Anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by WB. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at 1:2000 to detect 20 ug of whole cell lysate containing MK2.
TA342907 - MAPKAP Kinase-2
Host: Rabbit; Target Name: MAPKAPK2; Sample Tissue: A549 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
TA342908 - MAPKAP Kinase-2
WB Suggested Anti-MAPKAPK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA333242 - MAPKAP Kinase-2
Western blot analysis of extracts from Hela cells, using Phospho-MAPKAPK2-T334 antibody.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPKAP Kinase-2 antibody
AP32810PU-N  (0.5 µg/ml) staining of MEF lysates (35µg protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, IP, P, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
+3 additional images

OriGene recommends this product for Western blotting.

Highly validated antibody!

MAPKAP Kinase-2 (44-55) antibody
HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with AP32810PUN (0.5 μg/ml) in the left panel and with AP32811PU-N (0.5 μg/ml) in the right panel. Goat Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
+1 additional image

OriGene recommends this product for Western blotting.

MAPKAP Kinase-2 antibody
  Rabbit polyclonal MAPKAPK2 (Ab-272) antibody  
Western blot analysis of extracts from COS cells, using MAPKAPK2 (Ab-272) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MAPKAP Kinase-2 antibody
  Rabbit polyclonal MAPKAP Kinase 2 antibody  
Anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by WB. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at 1:2000 to detect 20 ug of whole cell lysate containing MK2. Rabbit Aff - Purified Chk, Hst, Hu, Ms, Rb, Rt, Xen, Ze E, IP, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

MAPKAP Kinase-2 antibody
  Phospho-MAPKAPK2-T334 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from Hela cells, using Phospho-MAPKAPK2-T334 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

MAPKAP Kinase-2 antibody
MAPKAP Kinase-2 Rabbit Aff - Purified Bov, Can, Hst, Hu, Ms, Rt IP, WB
0.1 mg / €380.00
  OriGene Technologies GmbH

MAPKAP Kinase-2 pThr334 antibody
MAPKAP Kinase-2 Rabbit Aff - Purified Hu WB
0.1 mg / €450.00
  OriGene Technologies GmbH

MAPKAP Kinase-2 pThr334 antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK-2 pThr334 antibody AP02538PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €300.00
  OriGene Technologies GmbH
+2 additional images

MAPKAP Kinase-2 pThr334 antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK-2 pThr334 antibody AP02538PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €230.00
  OriGene Technologies GmbH
+2 additional images

MAPKAP Kinase-2 antibody
Immunofluorescence staining of methanol-fixed HeLa cells using MAPKAPK-2 antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €300.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 antibody
Immunofluorescence staining of methanol-fixed HeLa cells using MAPKAPK-2 antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €230.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 antibody
Immunohistochemistry (IHC)analyzesof MAPKAPK-2 antibody (Cat.-No.: AP06619PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 pSer272 antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu Dot, P
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 antibody
Immunohistochemistry (IHC) analyzes of MAPKAPK-2 antibody (Cat.-No.: AP21090PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 antibody
Immunohistochemistry (IHC) analyzes of MAPKAPK-2 antibody (Cat.-No.: AP21135PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 pSer272 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody AP55823PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €300.00
  OriGene Technologies GmbH
+1 additional image

MAPKAP Kinase-2 pSer272 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody AP55823PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €230.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAPKAP Kinase-2 (transcript variant 2)
  Recombinant protein of human mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 2  
MAPKAP Kinase-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

MAPKAP Kinase-2 (transcript variant 1)
  Recombinant protein of human mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 1  
MAPKAP Kinase-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

MAPKAPK2 overexpression lysate
MAPKAPK2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

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21 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (2)
  2. Primary Antibody (17)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (17)
Target
  1. MAPKAP Kinase-2 (19)
Property
  1. 44-55 (1)
  2. pSer272 (3)
  3. pThr334 (3)
Reactivity
  1. Human (Hu) (17)
  2. Mouse (Ms) (15)
  3. Rat (Rt) (13)
  4. Hamster (Hst) (2)
  5. Bovine (Bov) (1)
  6. Canine (Can) (1)
  7. Chicken (Chk) (1)
  8. Rabbit (Rb) (1)
  9. Xenopus (Xen) (1)
  10. Zebrafish (Ze) (1)
Applications
  1. Western blot / Immunoblot (WB) (15)
  2. Paraffin Sections (P) (10)
  3. Enzyme Immunoassay (E) (4)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  5. Immunoprecipitation (IP) (4)
  6. Dot blot (Dot) (1)
Presentation
  1. Aff - Purified (15)
  2. Purified (2)
Host
  1. Rabbit (15)
  2. Goat (2)
Images
  1. 1 image (17)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (14)
  2. Paraffin Sections (P) (9)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  4. Immunoprecipitation (IP) (2)
  5. Dot blot (Dot) (1)
  6. IHC (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (21)
Service
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  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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