MAPRE3 antibody
Principal name
MAPRE3 antibody
Alternative names for MAPRE3 antibody
End-binding protein 3, EB1 protein family member 3, RP3, EB3, Microtubule-associated protein RP/EB family member 3
Ncbi ID
9606, NP_036458.2, NP_036458, NP_579928.1, NM_133350, NM_001007656
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MAPRE3 antibody
- Kit Component:
- - KN309780G1, Mapre3 gRNA vector 1 in pCas-Guide vector
- KN309780G2, Mapre3 gRNA vector 2 in pCas-Guide vector
- KN309780D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Komarova Y, Lansbergen G, Galjart N, Grosveld F, Borisy GG and Akhmanova A. 2005. EB1 and EB3 Control CLIP Dissociation from the Ends of Growing Microtubules. Mol. Biol. Cell. 16:5334-45.
Western blot analysis of MAPRE3 using MAPRE3 polyclonal antibody ( Cat # PAB0370 ) on 15 µgs of human brain lysate.
MAPRE3 MaxPab polyclonal antibody. Western Blot analysis of MAPRE3 expression in rat brain.
Western Blot analysis of MAPRE3 expression in transfected 293T cell line (H00022924-T01) by MAPRE3 MaxPab polyclonal antibody.Lane 1: MAPRE3 transfected lysate(30.91 KDa).Lane 2: Non-transfected lysate.
Immunofluorescent staining for ( A ) MAPRE3 over expressed COS-7 cells and ( B ) cell mitosis with MAPRE3 monoclonal antibody, clone KT36 ( Cat # MAB1677 ). Courtesy of Dr. Anna Akhmanova.
Anti-MAPRE3 (AP10053PU-N)(0.1µg/ml) staining of Human Brain (Amygdala) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Recombinant human MAPRE3, 1-281 aa, His-tagged
Western blot analysis of EB3 expression in SHSY5Y (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.
(0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, mouse brain tissue, Primary antibody: (MAPRE3 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MAPRE3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (MAPRE3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP10053PU-N | MAPRE3 antibody |
||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms | E, WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
|||||||
| TA311402 | MAPRE3 (151-164) antibody |
||||||
| Goat Anti-MAPRE3 (aa151-164) Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349912 | MAPRE3 antibody |
||||||
| Rabbit Polyclonal Anti-MAPRE3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AM20800PU-L | MAPRE3 antibody |
||||||
 |
Rat | IgG2a | KT53 | Aff - Purified | Hu | E, IP, WB |
1 mg /
€1,240.00
|
| OriGene Technologies GmbH | |||||||
| AM20800PU-N | MAPRE3 antibody |
||||||
|
Rat | IgG2a | KT53 | Aff - Purified | Hu | E, IP, WB |
0.2 mg /
€540.00
|
| OriGene Technologies GmbH | |||||||
| AM20800PU-S | MAPRE3 antibody |
||||||
|
Rat | IgG2a | KT53 | Aff - Purified | Hu | E, IP, WB |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304135 | MAPRE3 |
||||
| Recombinant protein of human microtubule-associated protein, RP/EB family, member 3 (MAPRE3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203812L2V | Lenti ORF particles, Mapre3 (GFP-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203812L1V | Lenti ORF particles, Mapre3 (Myc-DDK-tagged) - Mouse microtubule-associated protein, RP/EB family, member 3 (Mapre3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203870L2V | Lenti ORF particles, Mapre3 (GFP-tagged ORF) - Rat microtubule-associated protein, RP/EB family, member 3 (Mapre3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR203870L1V | Lenti ORF particles, Mapre3 (Myc-DDK-tagged ORF) - Rat microtubule-associated protein, RP/EB family, member 3 (Mapre3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||