MARCKS-like 1 antibody
Principal name
MARCKS-like 1 antibody
Alternative names for MARCKS-like 1 antibody
MARCKS-related protein, Macrophage myristoylated alanine-rich C kinase substrate, MARCKSL1, MLP, MRP
Ncbi ID
9606, NP_075385, NP_075385.1, NM_023009, NP_034937.1, NM_010807, NM_030862
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MARCKS-like 1 antibody
- Kit Component:
- - KN309794G1, Marcksl1 gRNA vector 1 in pCas-Guide vector
- KN309794G2, Marcksl1 gRNA vector 2 in pCas-Guide vector
- KN309794D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Jordanova, A., et al. Nat. Genet. 38(2):197-202(2006)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Immunoperoxidase of monoclonal antibody to MARCKSL1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Western Blot analysis of MARCKSL1 expression in transfected 293T cell line by MARCKSL1 monoclonal antibody (M02), clone 8C8.Lane 1: MARCKSL1 transfected lysate(19.398 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged MARCKSL1 is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of MARCKSL1 Antibody (C-term) Cat.-No AP52608PU-N in 293 cell line lysates (35ug/lane). This demonstrates the MARCKSL1 antibody detected the MARCKSL1 protein (arrow)
Human Tonsil, Germinal Center: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of MARCKSL1 expression in transfected 293T cell line by MARCKSL1 antibody Cat.-No. AM31898PU-N.
Detection limit for recombinant GST tagged MARCKSL1 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARCKSL1 (RC203181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARCKSL1.
ELISA: MARCKS like protein Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31898PU-N | MARCKS-like 1 (107-196) antibody |
||||||
 |
Mouse | IgG2b | 8C8 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
|||||||
| AP52608PU-N | MARCKS-like 1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411483 | MARCKSL1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219334L2V | Lenti ORF particles, Marcksl1 (GFP-tagged) - Mouse MARCKS-like 1 (Marcksl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR219334L1V | Lenti ORF particles, Marcksl1 (Myc-DDK-tagged) - Mouse MARCKS-like 1 (Marcksl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209302L2V | Lenti ORF particles, Marcksl1 (GFP-tagged ORF) - Rat MARCKS-like 1 (Marcksl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209302L1V | Lenti ORF particles, Marcksl1 (Myc-DDK-tagged ORF) - Rat MARCKS-like 1 (Marcksl1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||