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MARK2 antibody

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Principal name

MARK2 antibody

Alternative names for MARK2 antibody

Serine/threonine-protein kinase, 2, ELKL motif kinase, EMK1, PAR1 homolog, MARK2, EMK1, MAP/microtubule affinity-regulating kinase

SwissProt ID

O08679 (Rat), Q05512 (Mouse), Q7KZI7 (Human)

Gene ID

2011 (MARK2), 13728 (Mark2), 60328

Ncbi ID

9606, NP_001034557, NP_001034558.2, NP_001034558.1, NP_059672.2, NM_004954, NP_004945, NP_059672, NM_001080388, NM_001080389, NM_001080390, NM_021699, NM_017490, NM_001163297, NM_001163296, NM_001039469

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MARK2 antibody

MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule
stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

H00002011-M01 - MARK2
Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.
PAB3195 - MARK2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MARK2 polyclonal antibody ( Cat # PAB3195 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB3195 - MARK2
Western blot analysis of MARK2 polyclonal antibody ( Cat # PAB3195 ) in HepG2 cell line lysates. MARK2 ( arrow ) was detected using the purified polyclonal antibody.
PAB7578 - MARK2
MARK2 polyclonal antibody ( Cat # PAB7578 ) ( 0.3 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00002011-D01P - MARK2
Western Blot analysis of MARK2 expression in transfected 293T cell line (H00002011-T02) by MARK2 MaxPab polyclonal antibody.Lane 1: MARK2 transfected lysate(84.30 KDa).Lane 2: Non-transfected lysate.
H00002011-D01P - MARK2
MARK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of MARK2 expression in A-431.
AP14909PU-N - MARK2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP14909PU-N - MARK2
(LEFT) Western blot analysis of anti-EMK Antibody (C-term)in HepG2 cell line lysates. EMK-V581(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-EMK Antibody (C-term) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.
PAB10332 - MARK2
Western blot using MARK2 (phospho T595) polyclonal antibody ( Cat # PAB10332 ) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 ( arrowhead ).HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta ( lane 3 ) or pCMV-3 x Flag-MARK2 only ( lane 4 ).Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody ( Cat # PAB10332 ).An untransfected HEK293 cell lysate was used as a negative control ( lane 2 ).MW markers are shown for size comparison ( lane 1 ).PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta.Personnel Communication, S. Shaw, NCI, Center for Cancer Research.
AP09297PU-N - MARK2
Western blot using Affinity Purified anti-MARK2 pT595 antibody shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3xFlag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3xFlag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with anti-MARK2 pT595. An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phosphospecific staining are observed in lysates from cells transfected with both MARK2 and PKCzeta.
B1094-1 - MARK2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MARK2 (Ab-596) Antibody. The picture on the right is treated with the synthesized peptide.
B1094-1 - MARK2
Immunofluorescence analysis of HuvEc cells, using MARK2 (Ab-596) Antibody. The picture on the right is treated with the synthesized peptide.
C11249-1 - MARK2
Immunofluorescence analysis of A549 cells, using MARK2 Antibody. The picture on the right is treated with the synthesized peptide.
C11249-1 - MARK2
Western blot analysis of extracts from COS7 cells, using MARK2 Antibody. The lane on the right is treated with the synthesized peptide.
TP310279 - MARK2
TP760915 - MARK2
TA303346 - MARK2
TA303346 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA313814 - MARK2
Western blot analysis of extracts from COS-7 cells, using MARK2 antibody.The lane on the right is treated with the synthesized peptide.
TA313814 - MARK2
Immunofluorescence analysis of A549 cells, using MARK2 antibody.The picture on the right is treated with the synthesized peptide.

2 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MARK2 pThr595 antibody
Western blot using Affinity Purified anti-MARK2 pT595 antibody shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3xFlag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3xFlag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with anti-MARK2 pT595. An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phosphospecific staining are observed in lysates from cells transfected with both MARK2 and PKCzeta. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

MARK2 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

2 Item(s)

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Available Filters

Product Type
  1. Primary Antibody (2)
Target
  1. MARK2 (2)
Property
  1. C-term (1)
  2. pThr595 (1)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (2)
  3. Bovine (Bov) (1)
  4. Canine (Can) (1)
  5. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (2)
Service
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