MARK4 antibody
Principal name
MARK4 antibody
Alternative names for MARK4 antibody
MAP microtubule affinity-regulating kinase 4, MARKL1
Ncbi ID
9606, NP_001186796.1, NP_001186796, AAI66620.1, NP_113605.2, NM_031417, NM_172279
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of MARK4 antibody
- Kit Component:
- - KN222580G1, MARK4 gRNA vector 1 in pCas-Guide vector
- KN222580G2, MARK4 gRNA vector 2 in pCas-Guide vector
- KN222580D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kato T, Satoh S, Okabe H, Kitahara O, Ono K, Kihara C, Tanaka T, Tsunoda T, Yamaoka Y, Nakamura Y, Furukawa Y.
Isolation of a novel human gene, MARKL1, homologous to MARK3 and its involvement in hepatocellular carcinogenesis.
Neoplasia. 2001 Jan-Feb;3(1):4-9.
PMID: 11326310
MARK4 polyclonal antibody ( Cat # PAB6142 ) staining ( 0.5 µg/ml ) of human prostate lysate ( 35 µg protein in RIPA buffer ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
(LEFT)Western blot analysis of anti-MARK4 Pab in mouse liver tissue lysate (35ug/lane). MARK4(arrow) was detected using the purified Pab (1:40dilution). (RIGHT)Western blot analysis of MARK4 (arrow) using rabbit polyclonal hMARK4-R626 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of A549 cells, using MARK4 Antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemical analysis of MARK4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MARK4 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MARK4 expression in Jurkat (A); K562 (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808507)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MARK4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808507)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808507)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808507)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK4 (RC222580, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK4.
TA302535 (0.5µg/ml) staining of human prostate lysate (35µg protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunofluorescence analysis of A549 cells, using MARK4 antibody.The picture on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808507 | MARK4 antibody |
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| MARK4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7) | |||||||
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Mouse | IgG1 | OTI9B7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA302535 | MARK4 (C-term) antibody |
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| Goat Polyclonal Antibody against MARK4 | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA312424 | MARK4 antibody |
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| Rabbit polyclonal anti-MARK4 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP13785PU-N | MARK4 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP31612PU-N | MARK4 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY403112 | MARK4 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215461L2V | Lenti ORF particles, Mark4 (GFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), 200 uL, >10^7 TU/mL |
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200 µl /
€1,540.00
|
|
| OriGene Technologies, Inc. | ||
| MR215461L1V | Lenti ORF particles, Mark4 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,380.00
|
|
| OriGene Technologies, Inc. | ||