MARS antibody
Principal name
MARS antibody
Alternative names for MARS antibody
Methionyl-tRNA synthetase, cytoplasmic, methionine-tRNA synthetase, METRS, MTRNS
Ncbi ID
9606, NP_004981, NP_004981.2, NM_004990, NM_001003913, NM_001171582
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of MARS antibody
- Kit Component:
- - KN202932G1, MARS gRNA vector 1 in pCas-Guide vector
- KN202932G2, MARS gRNA vector 2 in pCas-Guide vector
- KN202932D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
Immunofluorescence of monoclonal antibody to MARS on HepG2 cell. [antibody concentration 10 ug/ml]
MARS monoclonal antibody (M01), clone 5G5 Western Blot analysis of MARS expression in HepG2 ( Cat # L019V1 ).
Immunofluorescence of purified MaxPab antibody to MARS on HeLa cell. [antibody concentration 10 ug/ml]
MARS MaxPab polyclonal antibody. Western Blot analysis of MARS expression in human pancreas.
Western Blot analysis of MARS expression in transfected 293T cell line (H00004141-T01) by MARS MaxPab polyclonal antibody.Lane 1: MARS transfected lysate(99 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Human HeLa; WB Suggested Anti-MARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate; MARS antibody - middle region (ARP32692_P050) in Human HeLa cells using Western Blot
Immunoprecipitation of MARS transfected lysate using anti-MARS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MARS MaxPab mouse polyclonal antibody (B01) (H00004141-B01).
WB Suggested Anti-MARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Hela cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP14681PU-N | MARS (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302932 | MARS |
||||
| Recombinant protein of human methionyl-tRNA synthetase (MARS) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417604 | MARS overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC202932L2V | Lenti ORF particles, MARS (mGFP-tagged) - Human methionyl-tRNA synthetase (MARS), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,010.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218764L2V | Lenti ORF particles, Mars (GFP-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,760.00
|
|
| OriGene Technologies, Inc. | ||
| MR211112L2V | Lenti ORF particles, Mars (GFP-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,750.00
|
|
| OriGene Technologies, Inc. | ||
| MR218764L1V | Lenti ORF particles, Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,580.00
|
|
| OriGene Technologies, Inc. | ||
| MR211112L1V | Lenti ORF particles, Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,570.00
|
|
| OriGene Technologies, Inc. | ||
