MCEE antibody
Principal name
MCEE antibody
Alternative names for MCEE antibody
mitochondrial Methylmalonyl-CoA epimerase, DL-methylmalonyl-CoA racemase
Ncbi ID
NP_115990, NM_032601, 9606, NP_115990.3, NP_082902.1, NM_028626
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of MCEE antibody
- Kit Component:
- - KN309864G1, Mcee gRNA vector 1 in pCas-Guide vector
- KN309864G2, Mcee gRNA vector 2 in pCas-Guide vector
- KN309864D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Yang GE., et al. (2009) Appl Biochem Biotechnol. 152(3):353-65.
Gradinger AB., et al. (2007) Hum Mutat. 28(10):1045.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with MCEE Antibody (C-term) Cat.-No AP52633PU-N, which peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of MCEE Antibody (C-term) Cat.-No AP52633PU-N in mouse liver tissue lysates (15ug/lane). This demonstrates the MCEE antibody detected MCEE protein (arrow).
Recombinant human MCEE, 37-176 aa, His-tagged
Human Brain; WB Suggested Anti-MCEE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; MCEE antibody - C-terminal region (ARP60330_P050) in Human Brain cells using Western Blot
Hum. Fetal Brain; MCEE antibody - C-terminal region (ARP60330_P050) in Hum. Fetal Brain cells using Western Blot
Western Blot analysis of MCEE expression in transfected 293T cell line (H00084693-T01) by MCEE MaxPab polyclonal antibody.Lane 1: MCEE transfected lysate(19.36 KDa).Lane 2: Non-transfected lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MCEE mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCEE mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCEE mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000)
WB Suggested Anti-MCEE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain
Hum. Fetal Brain
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808532 | MCEE antibody |
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| MCEE mouse monoclonal antibody, clone OTI1B5 (formerly 1B5) | |||||||
 |
Mouse | IgG2b | OTI1B5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
|
||||||
| TA808534 | MCEE antibody |
||||||
| MCEE mouse monoclonal antibody, clone OTI6A1 (formerly 6A1) | |||||||
 |
Mouse | IgG2a | OTI6A1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA808608 | MCEE antibody |
||||||
| MCEE mouse monoclonal antibody, clone OTI11G7 (formerly 11G7) | |||||||
 |
Mouse | IgG1 | OTI11G7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA808531 | MCEE antibody |
||||||
| MCEE mouse monoclonal antibody, clone OTI9E12 (formerly 9E12) | |||||||
 |
Mouse | IgG1 | OTI9E12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA808533 | MCEE antibody |
||||||
| MCEE mouse monoclonal antibody, clone OTI4A6 (formerly 4A6) | |||||||
 |
Mouse | IgG1 | OTI4A6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP52633PU-N | MCEE (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760134 | MCEE (full length, N-term HIS tag) |
||||
| Recombinant protein of human methylmalonyl CoA epimerase (MCEE), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403176 | MCEE overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201564L2V | Lenti ORF particles, Mcee (GFP-tagged) - Mouse methylmalonyl CoA epimerase (Mcee), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201564L1V | Lenti ORF particles, Mcee (Myc-DDK-tagged) - Mouse methylmalonyl CoA epimerase (Mcee), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||