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MCM3 antibody

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Principal name

MCM3 antibody

Alternative names for MCM3 antibody

RLF subunit beta, P102 protein, P1-MCM3, mini-chromosome maintenance protein 3, DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1

SwissProt ID

A4FUD9 (Bovin), P24279 (Yeast), P25205 (Human), P25206 (Mouse), Q5ZMN2 (Chick)

Gene ID

4172 (MCM3), 17215 (Mcm3)

Ncbi ID

9606, NP_002379, NP_002379.2, NM_002388, BC031700, NM_008563

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of MCM3 antibody

MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Shukla,A., (2005) Biochem. Biophys. Res. Commun. 329 (4), 1240-1245

H00004172-M01 - MCM3
Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml]
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in K-562 ( Cat # L009V1 ).
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in human colon.
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in Daoy.
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in A-431 ( Cat # L015V1 ).
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7 Western Blot analysis of MCM3 expression in COLO 320 HSR ( Cat # L020V1 ).
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in Raw 264.7(Cat # L024V1 ).
H00004172-M01 - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in HeLa ( Cat # L013V1 ).
H00004172-A01 - MCM3
MCM3 polyclonal antibody (A01), Lot # 050919JC01 Western Blot analysis of MCM3 expression in HepG2 ( Cat # L019V1 ).
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in K-562 ( Cat # L009V1 ).
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in human colon.
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in Daoy.
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in A-431 ( Cat # L015V1 ).
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7 Western Blot analysis of MCM3 expression in COLO 320 HSR ( Cat # L020V1 ).
H00004172-M01A - MCM3
MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in HeLa ( Cat # L013V1 ).
H00004172-M02 - MCM3
MCM3 monoclonal antibody (M02), clone 2A11 Western Blot analysis of MCM3 expression in Hela NE ( Cat # L013V3 ).
H00004172-M02 - MCM3
Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.
H00004172-M03 - MCM3
Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml]
H00004172-M03 - MCM3
MCM3 monoclonal antibody (M03), clone 3E1. Western Blot analysis of MCM3 expression in Hela NE ( Cat # L013V3 ).
H00004172-M03 - MCM3
MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa ( Cat # L013V1 ).
H00004172-M03 - MCM3
Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.
H00004172-M04 - MCM3
MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in human ovary cancer.
H00004172-M04 - MCM3
MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Y-79 ( Cat # L042V1 ).
H00004172-M04 - MCM3
MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Hela NE ( Cat # L013V3 ).
H00004172-M04 - MCM3
Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.
H00004172-M06 - MCM3
MCM3 monoclonal antibody (M06), clone 3E11. Western Blot analysis of MCM3 expression in human spleen.
ARP36644_P050 - MCM3
Human 721_B; WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. MCM3 is supported by BioGPS gene expression data to be expressed in 721_B.; MCM3 antibody - C-terminal region (ARP36644_P050) in Human 721_B cells using Western Blot
ARP36645_P050 - MCM3
Human Lung; MCM3 antibody - C-terminal region (ARP36645_P050) in Human Lung cells using Immunohistochemistry
ARP36645_P050 - MCM3
Human Jurkat; WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. MCM3 is supported by BioGPS gene expression data to be expressed in Jurkat.; MCM3 antibody - C-terminal region (ARP36645_P050) in Human Jurkat cells using Western Blot
AM26592AF-N - MCM3

Western blot analysis of MCM3 expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), Hep-II (6), HepG2 (7), A431 (8), NIH/3T3 (9) and WR19L (10) using AM26592AF-N.


TP300208 - MCM3
TA308761 - MCM3
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308761 diluted at 1:10000.
TA308761 - MCM3
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MCM3(TA308761) antibody at 1:500 dilution.
TA308761 - MCM3
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(TA308761) antibody at 1:500 dilution.
TA324263 - MCM3
Predicted band size: 91 kDa . Positive control: K562, Jurkat, hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: Hela cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA324263 - MCM3
Predicted cell location: Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using MCM3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
TA324264 - MCM3
Predicted band size: 91 kDa . Positive control: K562, Jurkat and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA324264 - MCM3
Predicted cell location: Nucleus. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using MCM3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
TA326905 - MCM3
Western blot analysis of extracts of various cell lines, using MCM3 antibody.
TA326905 - MCM3
Immunohistochemistry of paraffin-embedded human esophageal cancer using MCM3 antibody at dilution of 1:200 (400x lens).
TA326905 - MCM3
Immunohistochemistry of paraffin-embedded human lung cancer using MCM3 antibody at dilution of 1:200 (400x lens).
TA326905 - MCM3
Immunohistochemistry of paraffin-embedded rat brain using MCM3 antibody at dilution of 1:200 (400x lens).
TA326905 - MCM3
Immunofluorescence analysis of HeLa cell using MCM3 antibody. Blue: DAPI for nuclear staining.
TA327945 - MCM3
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
TA341682 - MCM3
WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in 721_B
TA341683 - MCM3
WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in Jurkat
TA341683 - MCM3
Human Lung
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11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM3 antibody
  MCM3 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MCM3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

MCM3 antibody
  Rabbit Polyclonal antibody to MCM3 (minichromosome maintenance complex component 3)  
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308761 diluted at 1:10000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

MCM3 antibody
  Rabbit polyclonal MCM3 (Thr722) antibody(Phospho-specific)  
MCM3 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

MCM3 antibody
  Anti-MCM3 Rabbit Polyclonal Antibody  
Predicted band size: 91 kDa . Positive control: K562, Jurkat, hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: Hela cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MCM3 antibody
  Anti-MCM3 Rabbit Polyclonal Antibody  
Predicted band size: 91 kDa . Positive control: K562, Jurkat and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MCM3 (full length) antibody
Western blot analysis of MCM3 expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), Hep-II (6), HepG2 (7), A431 (8), NIH/3T3 (9) and WR19L (10) using AM26592AF-N. Mouse IgG2a 3A2 Azide Free Hu ICC/IF, IP, P, WB
0.1 mg / €425.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MCM3
  Recombinant protein of human minichromosome maintenance complex component 3 (MCM3)  
MCM3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

MCM3 overexpression lysate
MCM3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mcm3 (GFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Mcm3 (GFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL  
200 µl / €1,630.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S, 200 uL, >10^7 TU/mL
Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S, 200 uL, >10^7 TU/mL  
200 µl / €1,330.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL  
200 µl / €1,460.00
  OriGene Technologies, Inc.
 

11 Item(s)

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11 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (3)
  3. Primary Antibody (6)
  4. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (1)
Target
  1. MCM3 (7)
Property
  1. full length (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (1)
  3. Azide Free (1)
Host
  1. Rabbit (5)
  2. Mouse (1)
Images
  1. 1 image (6)
  2. 3+ images (2)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (4)
  3. Immunofluorescence (IF) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (11)
Service
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