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MCM5 antibody

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Principal name

MCM5 antibody

Alternative names for MCM5 antibody

CDC46 homolog, P1-CDC46, CDC46, DNA replication licensing factor MCM5, minichromosome maintenance complex component 5

SwissProt ID

P29496 (Yeast), P33992 (Human), P49718 (Mouse), Q0V8B7 (Bovin)

Gene ID

4174 (MCM5)

Ncbi ID

NP_006730.2, NP_006730, 9606, AAH00142, NM_006739

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Chk (Chicken), Mky (Monkey), Rb (Rabbit), Eq (Equine)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of MCM5 antibody

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2 - 6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity; and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

General readings

Ohtani,K., et al., (1999) Oncogene 18 (14), 2299-2309

AP06217PU-N - MCM5
Immunohistochemistry (IHC) analyzes of MCM5 antibody (Cat.-No.: AP06217PU-N) in paraffin-embedded human tonsil tissue.
AP06217PU-N - MCM5
Western blot(WB) analysis of MCM5 antibody (Cat.-No.: AP06217PU-N) in extracts from HepG2 cells.
ARP36654_T100 - MCM5
Human HepG2; WB Suggested Anti-MCM5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; MCM5 antibody - N-terminal region (ARP36654_T100) in Human HepG2 cells using Western Blot
C0260-1 - MCM5
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using MCM5 Antibody. The picture on the right is treated with the synthesized peptide.
C0260-1 - MCM5
Immunofluorescence analysis of HepG2 cells, using MCM5 Antibody. The picture on the right is treated with the synthesized peptide.
C0260-1 - MCM5
Western blot analysis of extracts from HepG2 cells, using MCM5 Antibody. The lane on the right is treated with the synthesized peptide.
H00004174-M03A - MCM5
MCM5 monoclonal antibody (M03), clone 2H8. Western Blot analysis of MCM5 expression in Hela NE(Cat # L013V3 ).
SM1483P - MCM5
Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti huamn MCM5 (SM1483P/PT)
CPA1730-100ul - MCM5
Immunohistochemical analysis of CDC46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1730-100ul - MCM5
Immunofluorescent analysis of CDC46 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA1730-100ul - MCM5
Western blot analysis of CDC46 expression in HL60 (A); HeLa (B); HepG2 (C) whole cell lysates.
TA328021 - MCM5
Jurkat cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM5 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
TA332761 - MCM5
Western blot analysis of extracts of various cell lines, using MCM5 antibody.
TA332761 - MCM5
Immunofluorescence analysis of U20S cell using MCM5 antibody. Blue: DAPI for nuclear staining.
TA341689 - MCM5
WB Suggested Anti-MCM5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
TA347427 - MCM5
Western blot analysis on HepG2 cell lysate using MCM5 Antibody
TA312325 - MCM5
Western blot analysis of extracts from HepG2 cells, using MCM5 antibody (#TA312325).The lane on the right is treated with the synthesized peptide.
TA312325 - MCM5
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and cells (Lane 4), using MCM5 Antibody. The lane on the left is treated with systhesized peptide.
TA312325 - MCM5
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using MCM5 antibody (#TA312325).The picture on the right is treated with the synthesized peptide.
TA312325 - MCM5
Immunofluorescence analysis of HepG2 cells, using MCM5 antibody (#TA312325).The picture on the right is treated with the synthesized peptide.
TA312326 - MCM5
Western blot analysis of extracts from A549 cells, using MMP-11 antibody (#TA312326).The lane on the right is treated with the synthesized peptide.
TA312326 - MCM5
Western blot analysis of extracts from HepG2 cells (Lane 2) and HeLa cells (Lane 3), using MMP-11 Antibody. The lane on the left is treated with systhesized peptide.
TA312326 - MCM5
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP-11 antibody (#TA312326).The picture on the right is treated with the synthesized peptide.
TA312326 - MCM5
Immunofluorescence analysis of A549 cells, using MMP-11 antibody (#TA312326).The picture on the right is treated with the synthesized peptide.
TA349392 - MCM5
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: HepG2 cells, K562 cells, Jurkat cells, 231 cells, hela cells, Primary antibody: (MCM5 Antibody) at dilution 1/475, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
TA349392 - MCM5
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (MCM5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
TA349392 - MCM5
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (MCM5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
  1. Quick-Filters:
  2. Highly Validated (2)

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM5 antibody
  Rabbit polyclonal anti-MCM5 antibody  
Western blot analysis of extracts from HepG2 cells, using MCM5 antibody (#TA312325).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

MCM5 antibody
  Rabbit polyclonal anti-MMP-11 antibody  
Western blot analysis of extracts from A549 cells, using MMP-11 antibody (#TA312326).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

MCM5 antibody
  Rabbit anti-MCM5 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MCM5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

MCM5 antibody
  Rabbit Polyclonal Anti-MCM5 Antibody  
Western blot analysis on HepG2 cell lysate using MCM5 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MCM5 antibody
  Rabbit Polyclonal Anti-MCM5 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: HepG2 cells, K562 cells, Jurkat cells, 231 cells, hela cells, Primary antibody: (MCM5 Antibody) at dilution 1/475, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

MCM5 antibody
Immunohistochemistry (IHC) analyzes of MCM5 antibody (Cat.-No.: AP06217PU-N) in paraffin-embedded human tonsil tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

MCM5 antibody
Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti huamn MCM5 (SM1483P/PT) Mouse IgG2b CRCT5.1 Purified Hu C, P, WB
0.1 mg / €330.00
  OriGene Technologies GmbH

MCM5 antibody
Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti huamn MCM5 (SM1483P/PT) Mouse IgG2b CRCT5.1 Purified Hu C, P, WB
20 µg / €180.00
  OriGene Technologies GmbH

8 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (8)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (2)
Target
  1. MCM5 (8)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (6)
  3. Enzyme Immunoassay (E) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  5. Frozen sections (C) (2)
Presentation
  1. Purified (7)
  2. Aff - Purified (1)
Host
  1. Rabbit (6)
  2. Mouse (2)
Images
  1. 1 image (8)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Immunofluorescence (IF) (3)
  3. IHC (3)
  4. Paraffin Sections (P) (3)
Manufacturer
  1. OriGene Technologies (8)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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