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MCM6 antibody

Principal name

MCM6 antibody

Alternative names for MCM6 antibody

Mis5, P105MCM, minichromosome maintenance complex component 6, DNA replication licensing factor MCM6

SwissProt ID

P53091 (Yeast), P97311 (Mouse), Q14566 (Human), Q2KIZ8 (Bovin), Q62724 (Rat)

Gene ID

4175 (MCM6), 17219 (Mcm6)

Ncbi ID

NP_005906.2, 9606, NP_005906, NM_005915, NP_032593.1, NM_008567

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MCM6 antibody

The protein encoded by the MCM6 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

General readings

1. Bersaglieri, T., Sabeti, P. C., Patterson, N., Vanderploeg, T., Schaffner, S. F., Drake, J. A., Rhodes,
M., Reich, D. E., Hirschhorn, J. N. Genetic signatures of strong recent positive selection at the
lactase gene. Am. J. Hum. Genet. 74: 1111-1120, 2004.
2. Coelho, M., Luiselli, D., Bertorelle, G., Lopes, A. I., Seixas, S., Destro-Bisol, G., Rocha,
J. Microsatellite variation and evolution of human lactase persistence. Hum. Genet. 117: 329-339,
2005.
3. Enattah, N. S., Sahi, T., Savilahti, E., Terwilliger, J. D., Peltonen, L., Jarvela, I. Identification of a
variant associated with adult-type hypolactasia. Nature Genet. 30: 233-237, 2002.

Detection of human MCM6 in a RIPA extract of HeLa cells by Western Blotting using Rabbit anti Human MCM6 (SP2108P)

SP2108P

MCM6 polyclonal antibody (A01), Lot # 050919JC01 Western Blot analysis of MCM6 expression in SJCRH30 ( Cat # L027V1 ).

H00004175-A01

MCM6 polyclonal antibody (A01), Lot # ABNOVA060622QCS1. Western Blot analysis of MCM6 expression in U-2 OS.

H00004175-A01

Human Lung; MCM6 antibody - C-terminal region (ARP36650_T100) in Human Lung cells using Immunohistochemistry

ARP36650_T100

Human HepG2; WB Suggested Anti-MCM6 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; MCM6 antibody - C-terminal region (ARP36650_T100) in Human HepG2 cells using Western Blot

ARP36650_T100

Human Intestine; MCM6 antibody - C-terminal region (ARP36650_P050) in Human Intestine cells using Immunohistochemistry

ARP36650_P050

Human HepG2; WB Suggested Anti-MCM6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; MCM6 antibody - C-terminal region (ARP36650_P050) in Human HepG2 cells using Western Blot

ARP36650_P050

Immunoperoxidase of purified MaxPab antibody to MCM6 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]

H00004175-B01P

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC6 and MCM6 Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-MCM6 mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00004175-B01P

Immunofluorescence of purified MaxPab antibody to MCM6 on HeLa cell. [antibody concentration 10 ug/ml]

H00004175-B01P

Western Blot analysis of MCM6 expression in transfected 293T cell line (H00004175-T01) by MCM6 MaxPab polyclonal antibody.Lane 1: MCM6 transfected lysate(90.31 KDa).Lane 2: Non-transfected lysate.

H00004175-B01P

MCM6 MaxPab polyclonal antibody. Western Blot analysis of MCM6 expression in human placenta.

H00004175-B01P

Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue reacted with MCM6 Antibody (N-term) Cat.-No AP52640PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

AP52640PU-N

Western blot analysis of MCM6 Antibody (N-term) Cat.-No AP52640PU-N in 293 cell line lysates (35ug/lane). This demonstrates the MCM6 antibody detected the MCM6 protein (arrow).

AP52640PU-N

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)

TA590213

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)

TA590213

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)

TA590213

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MCM6 rabbit polyclonal antibody. (TA590213)

TA590213

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM6 (RC207097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM6.

TA590213

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MCM6 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

TA590213

ELISA: MCM6 Antibody

TA590213

TP307097

Western blot analysis of extracts of 293 cell lines , using MCM6 antibody.

TA327220

Immunohistochemistry of paraffin-embedded human lung using MCM6 antibody at dilution of 1:200 (400x lens).

TA327220

Immunohistochemistry of paraffin-embedded rat lung using MCM6 antibody at dilution of 1:200 (400x lens).

TA327220

Immunofluorescence analysis of U2OS cell using MCM6 antibody. Blue: DAPI for nuclear staining.

TA327220

Hela (left lane) and NIH-3T3 (right lane) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM6 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

TA328014

WB Suggested Anti-MCM6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate

TA341685

Human Intestine

TA341685

WB Suggested Anti-MCM6 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate

TA341686

Human Lung

TA341686

Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 293T cells, A549 cells, Raji cells, K562 cells, NIH/3T3 cells, Primary antibody: (MCM6 Antibody) at dilution 1/433.3, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

TA349393

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (MCM6 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

TA349393

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (MCM6 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

TA349393

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA327220	MCM6 antibody
	Rabbit anti-MCM6 Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / €335.00
						OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA590213	MCM6 antibody
	Rabbit Polyclonal MCM6 Antibody
	Rabbit	IgG	Purified	Can, Hu, Mky, Ms	E, P, WB	0.1 mg / €409.00
						OriGene Technologies, Inc.
+6 additional images	Highly validated antibody!
TA349393	MCM6 antibody
	Rabbit Polyclonal Anti-MCM6 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
AP52640PU-N	MCM6 (N-term) antibody
	Rabbit	Ig	Aff - Purified	Hu	P, WB	0.4 ml / €415.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP307097	MCM6
	Recombinant protein of human minichromosome maintenance complex component 6 (MCM6)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY401786	MCM6 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR210820L2V	Lenti ORF particles, Mcm6 (GFP-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), 200 uL, >10^7 TU/mL
		200 µl / €1,640.00
		OriGene Technologies, Inc.

MR210820L1V	Lenti ORF particles, Mcm6 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), 200 uL, >10^7 TU/mL
		200 µl / €1,470.00
		OriGene Technologies, Inc.

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