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MD-2 antibody

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Principal name

MD-2 antibody

Alternative names for MD-2 antibody

Lymphocyte antigen 96, ESOP-1, LY96, ESOP1, MD2

SwissProt ID

P58754 (Bovin), Q9JHF9 (Mouse), Q9Y6Y9 (Human)

Gene ID

23643 (LY96), 17087 (Ly96)

Ncbi ID

9606, NM_015364, NP_001182726.1, NP_056179, NP_001182726, NP_001153183.1, NM_016923, NM_001159711, NM_001195797

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Functional assay (FN), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MD-2 antibody

TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4.
MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomers and multimers (sMD-2). Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.

H00023643-B01 - MD-2
LY96 MaxPab polyclonal antibody. Western Blot analysis of LY96 expression in human liver.
H00023643-B01 - MD-2
Western Blot analysis of LY96 expression in transfected 293T cell line (H00023643-T01) by LY96 MaxPab polyclonal antibody.Lane 1: LY96 transfected lysate(17.6 KDa).Lane 2: Non-transfected lysate.
AP05225PU-N - MD-2
Western blot analysis of MD-2 in mouse spleen cell lysate with MD -2 antibody at 1 µg/ml.
H00023643-B01P - MD-2
LY96 MaxPab polyclonal antibody. Western Blot analysis of LY96 expression in human liver.
H00023643-B01P - MD-2
Western Blot analysis of LY96 expression in transfected 293T cell line (H00023643-T01) by LY96 MaxPab polyclonal antibody.Lane 1: LY96 transfected lysate(17.6 KDa).Lane 2: Non-transfected lysate.
H00023643-D01P - MD-2
LY96 MaxPab rabbit polyclonal antibody. Western Blot analysis of LY96 expression in mouse spleen.
H00023643-D01P - MD-2
Western Blot analysis of LY96 expression in transfected 293T cell line (H00023643-T01) by LY96 MaxPab polyclonal antibody.Lane 1: LY96 transfected lysate(18.40 KDa).Lane 2: Non-transfected lysate.
PAB10121 - MD-2
Western blot analysis of LY96 in mouse spleen tissue lysate with LY96 polyclonal antibody ( Cat # PAB10121 ) at 1 µg/ml.
MAB2752 - MD-2
Immunohistochemistry of LY96 in human spleen with LY96 monoclonal antibody, clone 9F1B1 ( Cat # MAB2752 ) at 2.5 µg/ml.
MAB2752 - MD-2
Western blot analysis of ( A ) 25 and ( B ) 125 ng of LY96 recombinant protein with LY96 monoclonal antibody, clone 9F1B1 ( Cat # MAB2752 ) at 1 µg/ml.
MAB2753 - MD-2
Immunohistochemistry of LY96 in human spleen with LY96 monoclonal antibody, clone 1A2E3 ( Cat # MAB2753 ) at 2.5 µg/ml.
MAB2753 - MD-2
Western blot analysis of ( A ) 25 and ( B ) 125 ng of LY96 recombinant protein with LY96 monoclonal antibody, clone 1A2E3 ( Cat # MAB2753 ) at 1 µg/ml.
ARP61217_P050 - MD-2
Human PANC1; WB Suggested Anti-LY96 Antibody. . Titration: 1.0 ug/ml. . Positive Control: PANC1 Whole Cell; LY96 antibody - C-terminal region (ARP61217_P050) in Human PANC1 cells using Western Blot
TP304686 - MD-2
TP330968 - MD-2
TA306161 - MD-2
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 ug/ml.
TA306161 - MD-2
Immunohistochemical staining of rat spleen cells using MD-2 antibody at 2 ug/ml.
TA306161 - MD-2
Immunofluorescence of MD-2 in Rat Spleen tissue with MD-2 antibody at 10 ug/mL.
TA319868 - MD-2
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/mL.
TA319868 - MD-2
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 ug/mL.
TA319868 - MD-2
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 ug/mL.
TA319869 - MD-2
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/mL.
TA319869 - MD-2
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 ug/mL.
TA332450 - MD-2
Western blot analysis of extracts of various cell lines, using LY96 antibody.
TA332450 - MD-2
Immunohistochemistry of paraffin-embedded human kidney using LY96 antibody at dilution of 1:100 (400x lens).
TA332450 - MD-2
Immunohistochemistry of paraffin-embedded human endometrial cancer using LY96 antibody at dilution of 1:100 (400x lens).
TA332450 - MD-2
Immunohistochemistry of paraffin-embedded human endometrium using LY96 antibody at dilution of 1:100 (200x lens).
TA336439 - MD-2
Immunohistochemistry: MD2 Antibody [TA336439] - IHC staining of normal human brain using 100-56655 (MD-2)at 10 ug/ml.
TA336439 - MD-2
Immunohistochemistry: MD2 Antibody [TA336439] - IHC staining of normal human brain using control (rabbit Ig) at 10 ug/ml.
TA336439 - MD-2
Immunohistochemistry-Paraffin: MD2 Antibody [TA336439] - analysis of MD2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.
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13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MD-2 antibody
  Rabbit anti-LY96 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using LY96 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

MD-2 antibody
  Rabbit Polyclonal MD-2 Antibody  
Western blot analysis of MD-2 in mouse spleen cell lysate with MD-2 antibody at 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

MD-2 (2-160) antibody
  Mouse MD-2 Monoclonal Antibody  
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/mL. Mouse IgG 9F1B1 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

MD-2 (2-160) antibody
  Mouse MD-2 Monoclonal Antibody  
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/mL. Mouse IgG 1A2E3 Aff - Purified Hu, Ms E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

MD-2 antibody
  Rabbit Polyclonal MD-2 Antibody  
Immunohistochemistry: MD2 Antibody [TA336439] - IHC staining of normal human brain using 100-56655 (MD-2)at 10 ug/ml. Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

MD-2 antibody
MD-2 Mouse IgG2b 18H10 FITC Hu FN
0.1 mg / €400.00
  OriGene Technologies GmbH

MD-2 antibody
MD-2 Mouse IgG2b 18H10 Purified Hu FN
0.1 mg / €350.00
  OriGene Technologies GmbH

MD-2 antibody
MD-2 Mouse IgG1 4H1 FITC Hu E, FN, ICC/IF, WB
0.1 mg / €430.00
  OriGene Technologies GmbH

MD-2 antibody
MD-2 Mouse IgG1 4H1 Purified Hu E, FN, ICC/IF, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

MD-2 antibody
Western blot analysis of MD-2 in mouse spleen cell lysate with MD -2 antibody at 1 µg/ml. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €600.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MD-2
  Recombinant protein of human lymphocyte antigen 96 (LY96)  
MD-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Published recently!

MD-2 (transcript variant 2)
  Purified recombinant protein of Homo sapiens lymphocyte antigen 96 (LY96), transcript variant 2.  
MD-2 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

LY96 overexpression lysate
LY96 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

13 Item(s)

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13 products - FILTER YOUR RESULTS!

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  1. Lysate (1)
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Target
  1. MD-2 (12)
Property
  1. 2-160 (2)
Reactivity
  1. Human (Hu) (10)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (5)
  3. Functional assay (FN) (4)
  4. Paraffin Sections (P) (4)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  6. Frozen sections (C) (2)
Presentation
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  2. Aff - Purified (2)
  3. FITC (2)
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  1. Human (2)
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  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (13)
Service
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