MED15 antibody
Principal name
MED15 antibody
Alternative names for MED15 antibody
ARC105, CTG7A, PCQAP, TIG1, TNRC7, Mediator complex subunit 1, Mediator of RNA polymerase II transcription subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, Trinucleotide repeat-containing gene 7 protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MED15 antibody
- Kit Component:
- - KN204224G1, MED15 gRNA vector 1 in pCas-Guide vector
- KN204224G2, MED15 gRNA vector 2 in pCas-Guide vector
- KN204224D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Thakur JK, Arthanari H, Yang F, Pan SJ, Fan X, Breger J, Frueh DP, Gulshan K, Li DK, Mylonakis E, Struhl K, Moye-Rowley WS, Cormack BP, Wagner G, Näär AM.
A nuclear receptor-like pathway regulating multidrug resistance in fungi.
Nature 2008 Apr 452 (7187): 604-9.
PMID: 18385733
Immunofluorescence of monoclonal antibody to PCQAP on HeLa cell. [antibody concentration 10 ug/ml]
PCQAP monoclonal antibody (M02), clone 4A4. Western Blot analysis of PCQAP expression in NIH/3T3(Cat # L018V1 ).
PCQAP monoclonal antibody (M02), clone 4A4 Western Blot analysis of PCQAP expression in Hela NE ( Cat # L013V3 ).
Detection of GAL11 by Western blotting using the GAL11 polyclonal antibody ( Cat # PAB1161 ) . Extract of budding yeast was using as antigen. The antiserum was diluted 5000 fold before use.
MED15 polyclonal antibody ( Cat # PAB11613 ) ( 1 µg/ml ) staining of human lung lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of MED15 expression in MCF7 (A); K562 (B); HeLa (C); NIH3T3 (D); PC12 (E) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807881)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807886)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807906)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807906)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807906)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807919)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MED15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MED15 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807950)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15.
TA305676 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (MED15 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807881 | MED15 antibody |
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| MED15 mouse monoclonal antibody, clone OTI2C6 (formerly 2C6) | |||||||
 |
Mouse | IgG1 | OTI2C6 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
||||||
| TA807919 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5) | |||||||
 |
Mouse | IgG1 | OTI1H5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA807950 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI1C11 (formerly 1C11) | |||||||
 |
Mouse | IgG1 | OTI1C11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
||||||
| TA305676 | MED15 (both isoforms) antibody |
||||||
| Goat Anti-PCQAP / MED15 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350538 | MED15 antibody |
||||||
| Rabbit Polyclonal Anti-MED15 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA807882 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) | |||||||
 |
Mouse | IgG1 | OTI2H6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA807883 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
 |
Mouse | IgG1 | OTI1A2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA807886 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5) | |||||||
 |
Mouse | IgG1 | OTI1D5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA807906 | MED15 antibody |
||||||
| MED15 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1) | |||||||
 |
Mouse | IgG1 | OTI3F1 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304224 | MED15 (transcript variant 2) |
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| Recombinant protein of human mediator complex subunit 15 (MED15), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402473 | MED15 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |