MED18 antibody
Principal name
MED18 antibody
Alternative names for MED18 antibody
Mediator complex subunit 18, p28b, Mediator of RNA polymerase II transcription subunit 18
Ncbi ID
NP_001120822, NP_001120822.1, NM_017638, NM_001127350, NP_060108, NP_080315.1, NM_026039
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MED18 antibody
- Kit Component:
- - KN201062G1, MED18 gRNA vector 1 in pCas-Guide vector
- KN201062G2, MED18 gRNA vector 2 in pCas-Guide vector
- KN201062D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MED18 expression in transfected 293T cell line (H00054797-T01) by MED18 MaxPab polyclonal antibody.Lane 1: MED18 transfected lysate(22.88 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MED18 expression in transfected 293T cell line (H00054797-T02) by MED18 MaxPab polyclonal antibody.Lane 1: MED18 transfected lysate(23.70 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of MED18 expression in HEK293T (A); mouse brain (B); rat kidney (C) whole cell lysates.
Western blot analysis of extracts from Jurkat cells and COLO cells, using MED18 antibody.The lane on the right is treated with the synthesized peptide.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Jurkat cell, Primary antibody: (MED18 Antibody) at dilution 1/400 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MED18 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315538 | MED18 antibody |
||||||
| Rabbit polyclonal anti-MED18 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350537 | MED18 antibody |
||||||
| Rabbit Polyclonal Anti-MED18 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301062 | MED18 (transcript variant 1) |
||||
| Recombinant protein of human mediator complex subunit 18 (MED18), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP325237 | MED18 (transcript variant 2) |
||||
| Recombinant protein of human mediator complex subunit 18 (MED18), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413638 | MED18 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY426751 | MED18 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202211L2V | Lenti ORF particles, Med18 (GFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) (Med18), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202211L1V | Lenti ORF particles, Med18 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) (Med18), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
