MED20 antibody
Principal name
MED20 antibody
Alternative names for MED20 antibody
TRFP, Mediator complex 20, Mediator of RNA polymerase II transcription subunit 20, TRF-proximal protein homolog
Ncbi ID
9606, NP_004266, NM_004275, NP_004266.2, BC060122, NM_020048, NM_001013178
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of MED20 antibody
- Kit Component:
- - KN203152G1, MED20 gRNA vector 1 in pCas-Guide vector
- KN203152G2, MED20 gRNA vector 2 in pCas-Guide vector
- KN203152D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sato S, Tomomori-Sato C, et al. (2004). Mol Cell. 14(5):685-91.Zhang X, Krutchinsky A, et al. (2011). Mol Cell. 19(1):89-100.
Immunofluorescence of purified MaxPab antibody to TRFP on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MED20 expression in transfected 293T cell line (H00009477-T01) by MED20 MaxPab polyclonal antibody.Lane 1: TRFP transfected lysate(23.32 KDa).Lane 2: Non-transfected lysate.
TRFP MaxPab polyclonal antibody. Western Blot analysis of TRFP expression in Hela NE.
WB Suggested Anti-MED20 Antibody; Titration: 1.0 ug/ml; Positive Control: RPMI-8226 Whole Cell
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303152 | MED20 |
||||
| Recombinant protein of human mediator complex subunit 20 (MED20) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401368 | MED20 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220310L2V | Lenti ORF particles, Med20 (GFP-tagged) - Mouse mediator complex subunit 20 (Med20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220310L1V | Lenti ORF particles, Med20 (Myc-DDK-tagged) - Mouse mediator complex subunit 20 (Med20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR200965L2V | Lenti ORF particles, Trfp (GFP-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR200965L1V | Lenti ORF particles, Trfp (Myc-DDK-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202486L2V | Lenti ORF particles, Med20 (GFP-tagged ORF) - Rat mediator complex subunit 20 (Med20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR202486L1V | Lenti ORF particles, Med20 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 20 (Med20), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||