MED21 antibody
Principal name
MED21 antibody
Alternative names for MED21 antibody
Mediator complex 21, SRB7, SURB7, Mediator of RNA polymerase II transcription subunit 21, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7
Gene ID
9412 (MED21)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MED21 antibody
- Kit Component:
- - KN202763G1, MED21 gRNA vector 1 in pCas-Guide vector
- KN202763G2, MED21 gRNA vector 2 in pCas-Guide vector
- KN202763D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Baumli,S., et al. (2005) J. Biol. Chem. 280 (18), 18171-18178Maldonado,E., et al. (1996) Nature 381 (6577), 86-89
Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10 ug/ml]
SURB7 monoclonal antibody (M04), clone 5A6 Western Blot analysis of SURB7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to SURB7 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.2 ug/ml]
Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10 ug/ml]
SURB7 monoclonal antibody (M08), clone 3E9 Western Blot analysis of SURB7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10 ug/ml]
SURB7 monoclonal antibody (M10), clone 2D9 Western Blot analysis of SURB7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SURB7 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10 ug/ml]
SURB7 monoclonal antibody (M03), clone 6B6 Western Blot analysis of SURB7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SURB7 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml]
SURB7 monoclonal antibody (M05), clone 4B5 Western Blot analysis of SURB7 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
Human Brain; WB Suggested Anti-MED21 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; MED21 antibody - middle region (ARP37735_P050) in Human Brain cells using Western Blot
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED21 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-MED21 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314905 | MED21 antibody |
||||||
| Rabbit polyclonal anti-MED21 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP32261PU-N | MED21 antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, P |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302763 | MED21 |
||||
| Recombinant protein of human mediator complex subunit 21 (MED21) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC202763L2V | Lenti ORF particles, MED21 (mGFP-tagged) - Human mediator complex subunit 21 (MED21), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||