MED4 antibody
Principal name
MED4 antibody
Alternative names for MED4 antibody
Mediator complex 4, ARC36, DRIP36, VDRIP, HSPC126, Mediator of RNA polymerase II transcription subunit 4, Vitamin D3 receptor-interacting protein complex 36 kDa component, Activator-recruited cofactor 36 kDa component, TRAP/SMCC/PC2 subunit p36 subunit
Ncbi ID
NP_054885, 9606, AAH05189, NP_054885.1, NP_080395.1, NM_026119, NM_001024256, NM_014166
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MED4 antibody
- Kit Component:
- - KN309945G1, Med4 gRNA vector 1 in pCas-Guide vector
- KN309945G2, Med4 gRNA vector 2 in pCas-Guide vector
- KN309945D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Panierakis C., et al. (2009) Int J Infect Dis.13 (6):e437-43.
Western Blot analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.Lane 1: MED4 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Human Testis; MED4 antibody - middle region (ARP50561_P050) in Human Testis cells using Immunohistochemistry
Human Testis; Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-MED4 antibody (ARP50561_P050); MED4 antibody - middle region (ARP50561_P050) in Human Testis cells using Immunohistochemistry
Human Fetal liver; WB Suggested Anti-MED4 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate; MED4 antibody - middle region (ARP50561_P050) in Human Fetal liver cells using Western Blot
Human Testis; MED4 antibody - C-terminal region (ARP50562_P050) in Human Testis cells using Immunohistochemistry
Human Testis; MED4 antibody - C-terminal region (ARP50562_P050) in Human Testis cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MED4 Antibody Titration: 0.5ug/ml. Positive Control: HepG2 cell lysate; MED4 antibody - C-terminal region (ARP50562_P050) in Human HepG2 cells using Western Blot
Western blot analysis of MED4 in human testis tissue lysate with MED4 polyclonal antibody ( Cat # PAB13053 ) at 0.5 µg/ml.
Recombinant human MED4, 1-270 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 µg/ml.
Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 ug/ml.
Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 ug/ml.
WB Suggested Anti-MED4 Antibody Titration: 1 ug/ml; Positive Control: Fetal liver cell lysate
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0 ug/ml using anti-MED4 antibody
Human Testis
WB Suggested Anti-MED4 Antibody Titration: 0.5 ug/ml; Positive Control: HepG2 cell lysate
Human Testis
Human Testis
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using (MED4 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (MED4 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306618 | MED4 antibody |
||||||
| Rabbit Polyclonal MED4 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA350536 | MED4 antibody |
||||||
| Rabbit Polyclonal Anti-MED4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP23857PU-N | MED4 (C-term) antibody |
||||||
 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302663 | MED4 |
||||
| Recombinant protein of human mediator complex subunit 4 (MED4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203582L2V | Lenti ORF particles, Med4 (GFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 4 homolog (yeast) (Med4), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203582L1V | Lenti ORF particles, Med4 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 4 homolog (yeast) (Med4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205273L2V | Lenti ORF particles, Med4 (GFP-tagged ORF) - Rat mediator complex subunit 4 (Med4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR205273L1V | Lenti ORF particles, Med4 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 4 (Med4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||