MED6 antibody
Principal name
MED6 antibody
Alternative names for MED6 antibody
Mediator complex 6, ARC33, Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, Renal carcinoma antigen NY-REN-28
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MED6 antibody
- Kit Component:
- - KN202504G1, MED6 gRNA vector 1 in pCas-Guide vector
- KN202504G2, MED6 gRNA vector 2 in pCas-Guide vector
- KN202504D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Pavri, R., et al. Mol. Cell 18(1):83-96(2005)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003)
Western blot analysis of MED6 Antibody (Center) Cat.-No AP52657PU-N in WiDr cell line lysates (35ug/lane).This demonstrates the MED6 antibody detected the MED6 protein (arrow).
Western blot analysis of MED6 Antibody (C-term) Cat.-No AP52658PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the MED6 antibody detected the MED6 protein (arrow).
Transfected 293T; WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Transfected 293T; MED6 antibody - middle region (ARP34219_P050) in Transfected 293T cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; MED6 antibody - middle region (ARP34219_P050) in HCT116 using CHIP
Hum. Fetal Brain; Host:Rabbit. Target Name:MED6. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; MED6 antibody - middle region (ARP34220_P050) in Hum. Fetal Brain cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:MED6. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; MED6 antibody - middle region (ARP34220_P050) in Hum. Fetal Muscle cells using Western Blot
Human 721_B; WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. MED6 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; MED6 antibody - middle region (ARP34220_P050) in Human 721_B cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; MED6 antibody - middle region (ARP34220_P050) in HCT116 using CHIP
Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T01) by MED6 MaxPab polyclonal antibody.Lane 1: MED6 transfected lysate(27.17 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T02) by MED6 MaxPab polyclonal antibody.Lane 1: MED6 transfected lysate(27.06 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T02) by MED6 MaxPab polyclonal antibody.Lane 1: MED6 transfected lysate(27.06 KDa).Lane 2: Non-transfected lysate.
MED6 MaxPab rabbit polyclonal antibody. Western Blot analysis of MED6 expression in mouse liver.
Western Blot analysis of MED6 expression in transfected 293T cell line (H00010001-T03) by MED6 MaxPab polyclonal antibody.Lane 1: MED6 transfected lysate(28.40 KDa).Lane 2: Non-transfected lysate.
MED6 MaxPab rabbit polyclonal antibody. Western Blot analysis of MED6 expression in HepG2.
Immunoperoxidase of monoclonal antibody to MED6 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
Western Blot analysis of MED6 expression in transfected 293T cell line by MED6 monoclonal antibody (M07), clone 4C2.Lane 1: MED6 transfected lysate(28.4 KDa).Lane 2: Non-transfected lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED6 (RC202504, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED6.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED6 (RC202504, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED6.(1:2000)
WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Transfected 293T
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Host:Rabbit; Target Name:MED6; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:MED6; Sample Tissue:Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate; MED6 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Host: Rabbit; Target Name: MED6; Sample Tissue: Esophagus Tumor lysates; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: MED6; Sample Tissue: Lung Tumor lysates; Antibody Dilution: 1.0 ug/ml
Rabbit Anti-MED6 Antibody; Paraffin Embedded Tissue: Human Lung cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808183 | MED6 antibody |
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| MED6 mouse monoclonal antibody, clone OTI3C9 (formerly 3C9) | |||||||
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Mouse | IgG1 | OTI3C9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808213 | MED6 antibody |
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| MED6 mouse monoclonal antibody, clone OTI3B9 (formerly 3B9) | |||||||
 |
Mouse | IgG2a | OTI3B9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP52657PU-N | MED6 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP52658PU-N | MED6 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760037 | MED6 (full length, N-term HIS tag) |
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| Recombinant protein of human mediator complex subunit 6 (MED6), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417284 | MED6 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |