MER2 antibody
Principal name
MER2 antibody
Alternative names for MER2 antibody
Meiotic recombination 2 protein, REC107, YJR021C, J1462
SwissProt ID
P21651 (Yeast)
Gene ID
853478 (REC107)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB)
Background of MER2 antibody
Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.
Western blot using MER2 (phospho S30) polyclonal antibody ( Cat # PAB11308 ) shows detection of phosphorylated MER2 in whole cell extracts.Cells were either wild type ( lane 1 ) or contained MER2 deletions ( lane 2 ).Extracts were prepared from cells 4 hr after initiation of meiosis.Proteins were obtained using TCA precipitation.The primary antibody was used at a 1 : 7,500 dilution.Secondary antibody was used at 1:5,000 dilution.Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using MER2 (phospho S30) polyclonal antibody ( Cat # PAB11308 ) shows detection of phos-phorylated MER2, but not phosphatase treated or mutant cells.Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody.Cells were harvested 4 hrs after the initiation of meiosis and therefore contain mostly phosphorylated MER2.Lane 2 contains the same preparation after treatment with phosphatase.Lane 3 contains MER2-S30A protein as a phosphorylation control.This antibody appears to be specificfor phosphorylated MER2 at the S30 position with negligible cross reactivity against unphosphorylated protein.The primary antibody was used at a 1 : 5,000 dilution.Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using MER2 polyclonal antibody ( Cat # PAB11356 ) shows detection of phosphorylated and unphosphorylated MER2 in wild type, phosphatase treated and mutant cells.Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated MER2.Lane 2 contains the same preparation after treatment with phosphatase.Lane 3 contains MER2-S30A protein as a phosphorylation control.This antibody is reactive with both phosphor-ylated and unphosphorylated MER2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.Personal Communication. MichaelLichten, NIH, CCR, Bethesda, MD.
Western blot using affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphor-ylated protein. The primary antibody was used at a 1:5,000 dilution.
Western blot using affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (delta). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution.
Western blot using affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP09316PU-N | MER2 pSer30 antibody |
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Rabbit | IgG | Aff - Purified | S. cerevisiae | E, WB |
0.1 mg /
€515.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP09317PU-N | MER2 (26-35) antibody |
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 |
Rabbit | IgG | Aff - Purified | S. cerevisiae | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies GmbH | |||||||