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Merlin antibody

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Principal name

Merlin antibody

Alternative names for Merlin antibody

Neurofibromin-2, NF2, SCH, Schwannomin, Schwannomerlin, Moesin-ezrin-radixin-like protein, Schwannomin, Schwannomerlin

SwissProt ID

P35240 (Human), P46662 (Mouse), P59750 (Papan), Q63648 (Rat)

Gene ID

4771 (NF2), 18016 (Nf2), 25744

Ncbi ID

9606, NP_000259.1, NP_000259, NP_035028.2, BC005442, NM_010898, NM_013193, NM_181830, NM_181832, NM_181833, NM_016418, NM_181825, NM_181828, NM_181831

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Merlin antibody

Neurofibromatosis type 2 (NF2) is a dominantly inherited disorder characterized by the occurance of bilateral vestibular schwannomas and other central nervous system tumors, including multiple meningiomas. NF2 occurs in about 1 of 40,000 live births. The NF2 gene is highly penetrant; NF2-affected individuals have a 95% chance of developing bilateral vestibular schwannomas. NF2 is distinct from NF1, which is characterized by an incidence of 1 in 4000, maps to chromosome 17 and encodes a protein designated neurofibromin, which is a large protein with a GAP domain. Genetic linkage studies of both sporadic and familial tumors suggest that NF2 is caused by inactivation of a tumor suppressor gene that maps on chromosome 22q12 and encodes a 595 amino acid protein whose function appears to be mediated by interaction with the cytoskeleton.

General readings

Gutmann DH, Wright DE, Geist RT, Snider WD (1995) Expression of the neurofibromatosis 2 (NF2) gene isoforms during rat embryonic development. Human Molecular Genetics 4:471-478.
Scherer SS, Gutmann DH (1997) Expression of the neurofibromatosis 2 tumor suppressor gene product, merlin, in Schwann cells. J. Neurosci. Res. 46:595-605.

AP02513PU-S - Merlin
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Merlin pSer518 antibody AP02513PU (Red).
AP02513PU-S - Merlin
Figure 1. Western blot analysis of extracts from HUVEC cells untreated or treated with IFN-alpha (100ng/ml, 15min), using Merlin antibody AP02759PU (Line 1 and 2) and Merlin pSer518 antibody AP02513PU (Line 3 and 4).
AP02759PU-S - Merlin
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Merlin antibody Cat.-No AP02759PU-N/PU-S.
AP02759PU-S - Merlin
Figure 2. Western blot analysis of extracts from Mouse Brain tissue and C6 cells using Merlin antibody Cat.-No AP02759PU-N/PU-S.
AP07606PU-N - Merlin
AP07606PU-N NF2 antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, cortex at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
H00004771-B02P - Merlin
Immunofluorescence of purified MaxPab antibody to NF2 on HeLa cell. [antibody concentration 10 ug/ml]
H00004771-B02P - Merlin
Western Blot analysis of NF2 expression in transfected 293T cell line (H00004771-T02) by NF2 MaxPab polyclonal antibody.Lane 1: NF2 transfected lysate(55.77 KDa).Lane 2: Non-transfected lysate.
AP08595PU-N - Merlin
AP08595PU-N at 5µg/ml staining NF2/Merlin in Zebrafish brain cortex by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
H00004771-D01 - Merlin
Immunoprecipitation of NF2 transfected lysate using anti-NF2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NF2 MaxPab mouse polyclonal antibody (B02) (H00004771-B02).
AP20757PU-N - Merlin
Western blot (WB) analyzes of Merlin antibody (Cat.-No.: AP20757PU-N) in extracts from HuvEc cells.
AP20869PU-N - Merlin
Western blot (WB) analyzes of p-NF2 antibody (Cat.-No.: AP20869PU-N) in extracts from Jurkat IFN cells.
A7150-1 - Merlin
Western blot analysis of extracts from HuvEc cells treated with IFN-alpha 1000U/ml 18h, using Merlin (Phospho-Ser518) Antibody. The lane on the right is treated with the synthesized peptide.
A8046-1 - Merlin
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Merlin (Phospho-Ser10) Antibody. The picture on the right is treated with the synthesized peptide.
A8046-1 - Merlin
Western blot analysis of extracts from NIH-3T3 cells treated with IFN 2500U/ml 30', using Merlin (Phospho-Ser10) Antibody. The lane on the right is treated with the synthesized peptide.
B7150-1 - Merlin
Western blot analysis of extracts from HuvEc cells, treated with IFN-α 1000U/ml 18h, using Merlin (Ab-518) Antibody. The lane on the right is treated with the synthesized peptide.
B8046-1 - Merlin
Western blot analysis of extracts from RAW264.7 cells, using Merlin (Ab-10) Antibody. The lane on the right is treated with the synthesized peptide.
CPA3281-100ul - Merlin
Immunohistochemical analysis of Merlin (pS518) staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3281-100ul - Merlin
Western blot analysis of Merlin (pS518) expression in MCF7 IFN-treated (A); Jurkat IFN-treated (B) whole cell lysates.
TA590014 - Merlin
Immunofluorescent staining of HeLa cells using anti-NF2 rabbit polyclonal antibody (TA590014).
TA590014 - Merlin
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NF2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA590014 - Merlin
Western Blot: Neurofibromin 2 Antibody - Western Blot was performed using affinity purified SEQer NF2 antibody, aa(477-576) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer NF2 antibody, aa(477-576) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
TA590014 - Merlin
ELISA: Neurofibromin 2 Antibody
CPA4477-100ul - Merlin
Western blot analysis of Merlin expression in HepG2 (A); MCF7 (B) whole cell lysates.
CPA4477-100ul - Merlin
Immunohistochemical analysis of Merlin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA4477-100ul - Merlin
Immunofluorescent analysis of Merlin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TA319136 - Merlin
Phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by WB. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
TA324866 - Merlin
Western blot analysis of Merlin Antibody (N-term) (Cat. #TA324866) in NCI-H460 cell line lysates (35ug/lane). NF2 (arrow) was detected using the purified Pab.
TA324866 - Merlin
Merlin Antibody (N-term) (Cat. #TA324866) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Merlin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TA324866 - Merlin
Confocal immunofluorescent analysis of Merlin Antibody (N-term)(Cat. #TA324866) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
TA325710 - Merlin
Western blot analysis of Merlin phosphorylation expression in IFN-a treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA325711 - Merlin
Western blot analysis of Merlin phosphorylation expression in IFN-a treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA310010 - Merlin
Western blot analysis of extracts from HUVEC cells untreated or treated with IFN-a (100ng/ml, 15min) using Merlin antibody (Lanes 1 and 2) and Merlin (phospho-Ser518) antibody (Lanes 3 and 4).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Merlin antibody
  Rabbit anti-NF2 (Merlin, Phospho-Ser518) polyclonal antibody(Phospho-specific)  
Western blot analysis of extracts from HUVEC cells untreated or treated with IFN-a (100ng/ml, 15min) using Merlin antibody (Lanes 1 and 2) and Merlin (phospho-Ser518) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €355.00
  OriGene Technologies, Inc.

Merlin pSer518 antibody
  Rabbit polyclonal NF2 phospho S518 antibody  
Phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by WB. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

Merlin (N-term) antibody
  Rabbit polyclonal Merlin Antibody (N-term)  
Western blot analysis of Merlin Antibody (N-term) (Cat. #TA324866) in NCI-H460 cell line lysates (35ug/lane). NF2 (arrow) was detected using the purified Pab. Rabbit Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

Merlin antibody
  Rabbit Polyclonal Merlin Antibody  
Western blot analysis of Merlin phosphorylation expression in IFN-a treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

Merlin antibody
  Rabbit Polyclonal Merlin (Ser518) Antibody (Phospho-specific)  
Western blot analysis of Merlin phosphorylation expression in IFN-a treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

Merlin antibody
  Phospho-NF2-S518 Rabbit Polyclonal Antibody  
Merlin Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

Merlin antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Merlin antibody Cat.-No AP02759PU-N/PU-S. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €300.00
  OriGene Technologies GmbH
+1 additional image

Merlin antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Merlin antibody Cat.-No AP02759PU-N/PU-S. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €230.00
  OriGene Technologies GmbH
+1 additional image

Merlin pSer518 antibody
AP07606PU-N NF2 antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, cortex at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms E, IP, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

Merlin antibody
AP08595PU-N at 5µg/ml staining NF2/Merlin in Zebrafish brain cortex by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Goat IgG Aff - Purified Hu, Ze E, P
50 µg / €425.00
  OriGene Technologies GmbH

Merlin antibody
Merlin Goat Aff - Purified Ze E, P
0.1 mg / €380.00
  OriGene Technologies GmbH

Merlin antibody
Western blot (WB) analyzes of Merlin antibody (Cat.-No.: AP20757PU-N) in extracts from HuvEc cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Merlin pSer518 antibody
Western blot (WB) analyzes of p-NF2 antibody (Cat.-No.: AP20869PU-N) in extracts from Jurkat IFN cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

Nf2 - mouse gene knockout kit via CRISPR
Nf2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nf2 - mouse gene knockout kit via CRISPR
Nf2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nf2 - mouse gene knockout kit via CRISPR
Nf2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nf2 - mouse gene knockout kit via CRISPR
Nf2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NF2 (mGFP-tagged)-Human neurofibromin 2 (merlin) (NF2), transcript variant 12, 200 uL, >10^7 TU/mL
Lenti ORF particles, NF2 (mGFP-tagged)-Human neurofibromin 2 (merlin) (NF2), transcript variant 12, 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nf2 (GFP-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nf2 (GFP-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053), 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Items 1 to 20 of 22 total

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22 products - FILTER YOUR RESULTS!

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