MERTK antibody
Principal name
MERTK antibody
Alternative names for MERTK antibody
C-mer, MER, Receptor tyrosine kinase MerTK, Proto-oncogene tyrosine-protein kinase MER
Ncbi ID
9606, NP_006334, NP_006334.2, NP_032613.1, NM_006343, NM_008587, NM_022943
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)
Background of MERTK antibody
In vertebrate, the photoreceptor cells are the basic sensory apparatus of the retina primarily converting photon energy absorbed from light into neuronal signals. The photoreceptor proximal outer segments are synthesized daily by cell bodies, and outer segment tips are removed with a circadian rhythm. The shed outer segments are endocytosed by adjacent retinal pigment epithelial (RPE) cells, and are recycled to photoreceptors (1). Mutation of a receptor tyrosine kinase gene, Mertk, in the Royal College of Surgeons (RCS) rat results in defective phagocytosis of photoreceptor outer segments by the retinal pigment epithelium (RPE) and retinal degeneration (2) resulting in to retinitis pigmentosa.
The receptor tyrosine kinase MERTK protein is a approximately 170-175 kDa (994 amino acids) protein expressed in long evans rat testis and RPEs. The MERTK protein has a tyrosine kinase domain closer to the C-terminal region and putative phosphorylation sites.
Formalin-fixed and paraffin-embedded human brain tissue reacted with MERTK polyclonal antibody ( Cat # PAB3367 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of MERTK polyclonal antibody ( Cat # PAB3367 ) in NIH/3T3 cell lysate. MERK ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human brain tissue reacted with MERK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-MERK Pab in NIH/3T3 cell lysate. MERK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis using MER antibody Cat.-No AM06188SU-N against full-length MER recombinant protein.
Western blot analysis of MERTK expression in Jurkat (A); HeLa (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of MERTK expression in HEK293T (A); Jurkat (B); U937 (C) whole cell lysates.
Representative Standard Curve
Representative Standard Curve
Western blot analysis of extracts from K562 cells and Jurkat cells, using MERTK antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from NIH-3T3 cells, using MER/TYRO3 (Ab-753/685)antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells (Lane 2), using MER/TYRO3 (Ab-753/685) Antibody. The lane on the left is treated with synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MERTK antibody at dilution 1/13, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MERTK antibody at dilution 1/13, on the right is treated with the fusion protein. (Original magnification: x200)
MER Antibody (Cat. #TA324995) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MER antibody detected the MER protein (arrow).
Formalin-fixed and paraffin-embedded human lymphoma reacted with MERTK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of U251 cell stained with MERTK Antibody(Cat#TA324995/SH090316F).U251 cells were incubated with MERTK primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .MERTK immunoreactivity is localized to Vesicles significantly.
Flow cytometric analysis of jurkat cells using MERTK Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-MERTK antibody (2µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using MERTK antibody.
Western Blot: MERTK Antibody [TA337021] - analysis of MERTK in human spleen lysate in the 1) absence 2) presence of immunizing peptide and 3) mouse spleen lysate using this antibody. 0 ug/ml, 2.0 ug/ml and 0.5 ug/ml respectively.
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Phospho-Tyr749/681) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312805 | MERTK antibody |
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| Rabbit polyclonal anti-MERTK antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312806 | MERTK antibody |
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| Rabbit polyclonal MER/SKY (Tyr749/681) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA312807 | MERTK antibody |
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| Rabbit polyclonal MER/TYRO3 (Ab-753/685) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA322506 | MERTK antibody |
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| Anti-MERTK Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322507 | MERTK antibody |
||||||
| Anti-MERTK Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324995 | MERTK antibody |
||||||
| Rabbit polyclonal MERTK Antibody | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA327756 | MERTK antibody |
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| MERTK Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA332689 | MERTK antibody |
||||||
| Rabbit anti-MERTK Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
