METT10D antibody
Principal name
METT10D antibody
Alternative names for METT10D antibody
Methyltransferase METT10D, Methyltransferase 10 domain-containing protein
Ncbi ID
NP_076991, AAH50603.1, NP_076991.3, NM_024086, NP_080473.1, NM_026197
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of METT10D antibody
- Kit Component:
- - KN309995G1, Mett10d gRNA vector 1 in pCas-Guide vector
- KN309995G2, Mett10d gRNA vector 2 in pCas-Guide vector
- KN309995D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sergiev,P.V., (2008) J. Mol. Biol. 375 (1), 291-300
Human 293T; WB Suggested Anti-METT10D Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. METTL16 is supported by BioGPS gene expression data to be expressed in HEK293T.; METT10D antibody - N-terminal region (ARP47674_P050) in Human 293T cells using Western Blot
Human Jurkat; WB Suggested Anti-METT10D Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; METT10D antibody - N-terminal region (ARP47675_P050) in Human Jurkat cells using Western Blot
MET10 Antibody (C-term) (Cat. #AP52670PU-N) western blot analysis in mouse bladder tissue lysates (15µg/lane).This demonstrates the MET10 antibody detected MET10 protein (arrow).
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504665)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504665)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504665)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-METT10D monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-METT10D mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504710)
Anti-METT10D mouse monoclonal antibody (TA504710) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY METT10D(RC208648).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-METT10D monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D.
Anti-METT10D mouse monoclonal antibody (TA504728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY METT10D(RC208648).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D (RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D.
HEK293T cells transfected with either RC208648 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-METT10D antibody(TA504728), and then analyzed by flow cytometry.
WB Suggested Anti-METT10D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate.METTL16 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-METT10D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name: METTL16; Sample Tissue: Esophagus Tumor lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504665 | METT10D antibody |
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| METT10D mouse monoclonal antibody, clone OTI3E8 (formerly 3E8) | |||||||
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Mouse | IgG1 | OTI3E8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA504710 | METT10D antibody |
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| METT10D mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) | |||||||
 |
Mouse | IgG1 | OTI3B5 | Purified | Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
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| TA504697 | METT10D antibody |
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| METT10D mouse monoclonal antibody, clone OTIF10 (formerly F10) | |||||||
 |
Mouse | IgG2b | OTIF10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504728 | METT10D antibody |
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| METT10D mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
 |
Mouse | IgG1 | OTI1A2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP52670PU-N | METT10D (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308648 | METT10D |
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| Recombinant protein of human methyltransferase 10 domain containing (METT10D) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411358 | METTL16 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208777L2V | Lenti ORF particles, Mettl16 (GFP-tagged) - Mouse methyltransferase like 16 (Mettl16), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| MR208777L1V | Lenti ORF particles, Mettl16 (Myc-DDK-tagged) - Mouse methyltransferase like 16 (Mettl16), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||