MFAP4 antibody
Principal name
MFAP4 antibody
Alternative names for MFAP4 antibody
Microfibril-associated glycoprotein 4
Ncbi ID
9606, NP_002395, NM_002404, NP_001185624, NP_001185624.1, NP_083844.1, NM_001198695
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MFAP4 antibody
- Kit Component:
- - KN310027G1, Mfap4 gRNA vector 1 in pCas-Guide vector
- KN310027G2, Mfap4 gRNA vector 2 in pCas-Guide vector
- KN310027D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lausen M, et al. (1999) J Biol Chem. 274(45):32234-40.Zhao, Z., et al. (1995) Mol. Genet. 4: 589-597.
Detection limit for recombinant GST tagged MFAP4 is approximately 1ng/ml as a capture antibody.
Mouse; . Sample Type :. Mouse sciatic nerve. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Biotinylated Anti-Rabbit 1:1000 followed by avidin-biotin and diaminobenzidine. Secondary Antibody Dilution :. 1:1000. Gene Name :. MFAP4. Submitted by :. Beth Friedman, Ph.D., Project Scientist, Department of Pharmacology, UCSD School of Medicine, San Diego, CA; MFAP4 antibody - N-terminal region (ARP41500_P050) in Mouse cells using Immunohistochemistry
Human MCF-7; WB Suggested Anti-MFAP4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; MFAP4 antibody - N-terminal region (ARP41500_P050) in Human MCF-7 cells using Western Blot
The extract of lung (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MFAP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM50053PU-N | MFAP4 antibody |
||||||
 |
Mouse | IgG1 | AT12D11 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50053PU-S | MFAP4 antibody |
||||||
|
Mouse | IgG1 | AT12D11 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308999 | MFAP4 |
||||
| Recombinant protein of human microfibrillar-associated protein 4 (MFAP4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP331122 | MFAP4 (transcript variant 1) |
||||
| Recombinant protein of human microfibrillar-associated protein 4 (MFAP4), transcript variant 1. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419346 | MFAP4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC208999L2V | Lenti ORF particles, MFAP4 (mGFP-tagged) - Human microfibrillar-associated protein 4 (MFAP4), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
