MGEA5 antibody
Principal name
MGEA5 antibody
Alternative names for MGEA5 antibody
HEXC, KIAA0679, MEA5, Meningioma-expressed antigen 5, Bifunctional protein NCOAT, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Ncbi ID
9606, NP_001135906, NM_012215, NP_036347, NP_001135906.1, NP_076288.1, NM_023799, NM_131904, NM_001142434
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MGEA5 antibody
- Kit Component:
- - KN310064G1, Mgea5 gRNA vector 1 in pCas-Guide vector
- KN310064G2, Mgea5 gRNA vector 2 in pCas-Guide vector
- KN310064D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MGEA5 expression in transfected 293T cell line (H00010724-T01) by MGEA5 MaxPab polyclonal antibody.Lane1:MGEA5 transfected lysate(100.76 KDa).Lane2:Non-transfected lysate.
Immunoprecipitation of MGEA5 transfected lysate using anti-MGEA5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MGEA5 MaxPab mouse polyclonal antibody (B01) (H00010724-B01).
Predicted band size: 103 kDa . Positive control: Human fetal liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MGEA5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 103 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human lung cancer tissue . Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using MGEA5 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
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- Cited (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323894 | MGEA5 antibody |
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| Anti-MGEA5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323895 | MGEA5 antibody |
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| Anti-MGEA5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322411 | MGEA5 (transcript variant 1) |
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| Recombinant protein of human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY415892 | MGEA5 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC222411L2V | Lenti ORF particles, MGEA5 (mGFP-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,030.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211167L2V | Lenti ORF particles, Mgea5 (GFP-tagged) - Mouse meningioma expressed antigen 5 (hyaluronidase) (Mgea5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,770.00
|
|
| OriGene Technologies, Inc. | ||
| MR211167L1V | Lenti ORF particles, Mgea5 (Myc-DDK-tagged) - Mouse meningioma expressed antigen 5 (hyaluronidase) (Mgea5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,590.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211943L1V | Lenti ORF particles, Mgea5 (Myc-DDK-tagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,590.00
|
|
| OriGene Technologies, Inc. | ||
