MGMT antibody
Principal name
MGMT antibody
Alternative names for MGMT antibody
Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C), Flow Cytometry (F), Immunoprecipitation (IP)
Background of MGMT antibody
MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.
General readings
Svrcek, M., et al. Gut 59(11):1516-1526 (2010)
Palli, D., et al. Mutagenesis 25(6):569-575 (2010)
Kim, M., et al. Cancer Sci. 101(11):2436-2442 (2010)
Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010)
Jacob, R., et al. Anticancer Res. 30(7):2561-2566 (2010)
Immunoperoxidase of monoclonal antibody to MGMT on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to MGMT on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MGMT expression in transfected 293T cell line (H00004255-T01) by MGMT MaxPab polyclonal antibody.Lane 1: MGMT transfected lysate(22.77 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to MGMT on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MGMT expression in transfected 293T cell line (H00004255-T01) by MGMT MaxPab polyclonal antibody.Lane 1: MGMT transfected lysate(22.77 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of MGMT in CEM whole cell extract using MGMT monoclonal antibody, clone MT 23.2 ( Cat # MAB2383 ) at 1 : 100 dilution.
MGMT MaxPab rabbit polyclonal antibody. Western Blot analysis of MGMT expression in mouse liver.
Western Blot analysis of MGMT expression in transfected 293T cell line (H00004255-T01) by MGMT MaxPab polyclonal antibody.Lane 1: MGMT transfected lysate(21.60 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of Mgmt expression in 3T3cell lysate.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT monoclonal antibody, clone MT3.1 ( Cat # MAB3442 ).
Western blot analysis of Raji cell line with MGMT monoclonal antibody, clone MT3.1 ( Cat # MAB3442 ).
MGMT MaxPab rabbit polyclonal antibody. Western Blot analysis of MGMT expression in mouse liver.
Immunoprecipitation of MGMT transfected lysate using anti-MGMT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MGMT purified MaxPab mouse polyclonal antibody (B01P) (H00004255-B01P).
Western blot (WB) analysis of MGMT antibody (Cat.-No.: AP20557PU-N) in extracts from Jurkat cells.
ELISA: MGMT Antibody
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800732)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800732)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800732)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (TA800732) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (TA800732) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800856)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800856)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800856)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MGMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809118)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809120)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809129)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809130)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809131)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MGMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809132)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT.(1:2000)
Western Blot: MGMT Antibody (MT 23.2) - Western blot analysis of MGMT (MT 23.2) antibody - Detection of MGMT in MCF7 lysate using anti-MGMT at 1:1000 dilution.
Western Blot: MGMT antibody (MT 23.2) - Detection of MGMT in CEM whole cell extract using anti-MGMT at 1:100 dilution.
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells using anti-MGMT at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
MGMT Antibody (N-term) (Cat. #TA324503) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
MGMT Antibody (N-term) (Cat. #TA324503) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MGMT Antibody (C-term) (Cat. #TA324927) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MGMT Antibody(Cat. #TA325178) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
MGMT Antibody (Cat. #TA328166) western blot analysis in MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
Western blot analysis of MGMT expression in Jurkat cells.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM800006 | MGMT antibody |
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| MGMT mouse monoclonal antibody,clone UMAB52 | |||||||
 |
Mouse | IgG1 | UMAB52 | Purified | Hu, Mky | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
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| UM800007 | MGMT antibody |
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| MGMT mouse monoclonal antibody,clone UMAB56 | |||||||
 |
Mouse | IgG1 | UMAB56 | Purified | Hu, Mky | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| UM870006 | MGMT antibody |
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| MGMT mouse monoclonal antibody,clone UMAB52 | |||||||
|
Mouse | IgG1 | UMAB52 | Purified | Hu, Mky | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| UM870007 | MGMT antibody |
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| MGMT mouse monoclonal antibody,clone UMAB56 | |||||||
|
Mouse | IgG1 | UMAB56 | Purified | Hu, Mky | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA800732 | MGMT antibody |
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| MGMT mouse monoclonal antibody, clone OTIB12 (formerly B12) | |||||||
 |
Mouse | IgG1 | OTIB12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA800856 | MGMT antibody |
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| MGMT mouse monoclonal antibody, clone OTI4E6 (formerly 4E6) | |||||||
 |
Mouse | IgG1 | OTI4E6 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA809118 | MGMT antibody |
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| MGMT mouse monoclonal antibody, clone OTI6C4 (formerly 6C4) | |||||||
 |
Mouse | IgG1 | OTI6C4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
||||||
| TA809120 | MGMT antibody |
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| MGMT mouse monoclonal antibody, clone OTI5A7 (formerly 5A7) | |||||||
 |
Mouse | IgG1 | OTI5A7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
||||||
| TA809129 | MGMT antibody |
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| MGMT mouse monoclonal antibody, clone OTI2E2 (formerly 2E2) | |||||||
 |
Mouse | IgG1 | OTI2E2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
||||||
| TA809130 | MGMT antibody |
||||||
| MGMT mouse monoclonal antibody, clone OTI4F9 (formerly 4F9) | |||||||
 |
Mouse | IgG1 | OTI4F9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
||||||
| TA809131 | MGMT antibody |
||||||
| MGMT mouse monoclonal antibody, clone OTI2F10 (formerly 2F10) | |||||||
 |
Mouse | IgG1 | OTI2F10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
||||||
| TA809132 | MGMT antibody |
||||||
| MGMT mouse monoclonal antibody, clone OTI10H9 (formerly 10H9) | |||||||
 |
Mouse | IgG1 | OTI10H9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
||||||
| TA309748 | MGMT antibody |
||||||
| Mouse Monoclonal MGMT Antibody (MT 23.2) | |||||||
 |
Mouse | IgG2b | MT 23.2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA324503 | MGMT (N-term) antibody |
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| Rabbit polyclonal MGMT Antibody (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324927 | MGMT antibody |
||||||
| Rabbit polyclonal MGMT Antibody (C-term) | |||||||
 |
Rabbit | IgG | Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA325178 | MGMT antibody |
||||||
| Mouse monoclonal MGMT Antibody | |||||||
 |
Mouse | IgG1 | 888CT22.2.4 | Purified | Hu | WB |
0.4 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| TA328166 | MGMT antibody |
||||||
| Mouse monoclonal anti-MGMT antibody(Ascites) | |||||||
 |
Mouse | IgG1 | 888CT22.2.4 | Ascites | Hu | WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| TA347960 | MGMT antibody |
||||||
| Rabbit Polyclonal Anti-MGMT Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA800745 | MGMT antibody |
||||||
| MGMT mouse monoclonal antibody, clone OTID5 (formerly D5) | |||||||
 |
Mouse | IgG1 | OTID5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP20557PU-N | MGMT antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
