MICAL1 antibody
Principal name
MICAL1 antibody
Alternative names for MICAL1 antibody
MICAL, NICAL, NEDD9-interacting protein with calponin homology and LIM domain
Ncbi ID
9606, NM_001159291, NP_073602.2, NP_001152763.1, NM_022765, NP_073602, NM_138315, NM_001164433
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MICAL1 antibody
- Kit Component:
- - KN208308G1, MICAL1 gRNA vector 1 in pCas-Guide vector
- KN208308G2, MICAL1 gRNA vector 2 in pCas-Guide vector
- KN208308D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MICAL1 expression in transfected 293T cell line (H00064780-T01) by MICAL1 MaxPab polyclonal antibody.Lane 1: MICAL1 transfected lysate(107.91 KDa).Lane 2: Non-transfected lysate.
Human Placenta; WB Suggested Anti-MICAL1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Placenta; MICAL1 antibody - C-terminal region (ARP61957_P050) in Human Placenta cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Flow cytometric Analysis of Hela cells, using anti-MICAL1 antibody(TA501841),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
Anti-MICAL1 mouse monoclonal antibody (TA501842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501842),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501842), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501843), and then analyzed by flow cytometry.
Anti-MICAL1 mouse monoclonal antibody (TA501844) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501844), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody (TA501845) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501845) at 1:150 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501845), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-MICAL1 antibody(TA501845),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501846)
Anti-MICAL1 mouse monoclonal antibody (TA501846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501846), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501846),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-MICAL1 mouse monoclonal antibody (TA501847) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501847), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501848)
Anti-MICAL1 mouse monoclonal antibody (TA501848) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
Flow cytometric Analysis of Hela cells, using anti-MICAL1 antibody(TA501848),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501848),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501848), and then analyzed by flow cytometry.
Anti-MICAL1 mouse monoclonal antibody (TA501849) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501849), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501893)
Anti-MICAL1 mouse monoclonal antibody (TA501893) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MICAL1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501893), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501893),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-MICAL1 mouse monoclonal antibody (TA501894) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501894), and then analyzed by flow cytometry.
- Quick-Filters:
- Highly Validated (7)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501841 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2) | |||||||
 |
Mouse | IgG1 | OTI1C2 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +10 additional images |
Highly validated antibody!
|
||||||
| TA501842 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI6H4 (formerly 6H4) | |||||||
 |
Mouse | IgG1 | OTI6H4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
||||||
| TA501845 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI6H10 (formerly 6H10) | |||||||
 |
Mouse | IgG1 | OTI6H10 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA501846 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG2b | OTI2H1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
||||||
| TA501847 | MICAL1 antibody |
||||||
| anti-MICAL1 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1) | |||||||
 |
Mouse | IgG2a | OTI4D1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA501848 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1) | |||||||
 |
Mouse | IgG2b | OTI4A1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA501893 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI4B1 (formerly 4B1) | |||||||
 |
Mouse | IgG1 | OTI4B1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA501843 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3) | |||||||
 |
Mouse | IgG1 | OTI5C3 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA501844 | MICAL1 antibody |
||||||
| anti-MICAL1 mouse monoclonal antibody, clone OTI6G2 (formerly 6G2) | |||||||
 |
Mouse | IgG1 | OTI6G2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA501849 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI6C7 (formerly 6C7) | |||||||
 |
Mouse | IgG1 | OTI6C7 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA501894 | MICAL1 antibody |
||||||
| MICAL1 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6) | |||||||
 |
Mouse | IgG1 | OTI3C6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308308 | MICAL1 |
||||
| Recombinant protein of human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402940 | MICAL1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY432077 | MICAL1 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211527L2V | Lenti ORF particles, Mical1 (GFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,950.00
|
|
| OriGene Technologies, Inc. | ||
| MR211527L1V | Lenti ORF particles, Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,760.00
|
|
| OriGene Technologies, Inc. | ||
| MR218242L1V | Lenti ORF particles, Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,670.00
|
|
| OriGene Technologies, Inc. | ||
