MID2 / RNF60 antibody
Principal name
MID2 / RNF60 antibody
Alternative names for MID2 / RNF60 antibody
Midin-2, Midline defect 2, Midline-2, RING finger protein 60, FXY2, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Tripartite motif-containing protein 1
Ncbi ID
9606, NP_036348.2, NM_052817, NM_012216, NP_036348, NP_035975.1, NM_011845
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MID2 / RNF60 antibody
- Kit Component:
- - KN310090G1, Mid2 gRNA vector 1 in pCas-Guide vector
- KN310090G2, Mid2 gRNA vector 2 in pCas-Guide vector
- KN310090D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.
MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407. PMID: 10400986
Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T01) by MID2 MaxPab polyclonal antibody.Lane 1: MID2 transfected lysate(75.35 KDa).Lane 2: Non-transfected lysate.
MID2 polyclonal antibody ( Cat # PAB6327 ) staining ( 1 µg/ml ) of mouse heart lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.Lane 1: MID2 transfected lysate(75.35 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MID2 expression in transfected 293T cell line (H00011043-T02) by MID2 MaxPab polyclonal antibody.Lane 1: MID2 transfected lysate(75.35 KDa).Lane 2: Non-transfected lysate.
Kidney: Fromalin-Fixed Paraffin-Embedded (FFPE)
Antibody (1 µg/ml) staining of mouse heart lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
TA302853 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302853 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB Suggested Anti-Mid2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Small Intestine
Host: Rabbit; Target Name: MID2; Sample Tissue: NCI-H226 Whole Cell lysates; Antibody Dilution: 1.0 ug/mlMID2 is supported by BioGPS gene expression data to be expressed in NCIH226
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302853 | MID2 / RNF60 (C-term) antibody |
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| Goat Polyclonal Antibody against MID2 | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP08553PU-N | MID2 / RNF60 (723-735) antibody |
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 |
Goat | IgG | Aff - Purified | Elephant, Hu, Mky, Ms | E, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY409474 | MID2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC224217L2V | Lenti ORF particles, MID2 (mGFP-tagged)-Human midline 2 (MID2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,669.00
|
|
| OriGene Technologies, Inc. | ||
| RC224217L1V | Lenti ORF particles, MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,504.00
|
|
| OriGene Technologies, Inc. | ||
| RC229356L1V | Lenti ORF particles, MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,550.00
|
|
| OriGene Technologies, Inc. | ||