MIER2 antibody
Principal name
MIER2 antibody
Alternative names for MIER2 antibody
KIAA1193, Mi-er2, Mesoderm induction early response protein 2
Ncbi ID
9606, NP_060020, NP_060020.1, NM_017550, NM_027422, NP_081698.2
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MIER2 antibody
- Kit Component:
- - KN310096G1, Mier2 gRNA vector 1 in pCas-Guide vector
- KN310096G2, Mier2 gRNA vector 2 in pCas-Guide vector
- KN310096D, donor vector containing left and right homologous arms and RFP-BSD functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Human Stomach; WB Suggested Anti-MIER2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; MIER2 antibody - middle region (ARP56973_P050) in Human Stomach cells using Western Blot
Human Jurkat; WB Suggested Anti-MIER2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; MIER2 antibody - middle region (ARP56974_P050) in Human Jurkat cells using Western Blot
Western blot analysis in NCI-H460 cell line lysates (35ug/lane) using MIER2 antibody Cat.-No. AP52697PU-N (N-term). This demonstrates the MIER2 antibody detected the MIER2 protein (arrow).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) using MIER2 antibody Cat.-No. AP52697PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MIER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504260)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MIER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504260)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MIER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504260)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MIER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504260)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIER2 (RC207065, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIER2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MIER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504403)
Anti-MIER2 mouse monoclonal antibody (TA504403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIER2(RC207065).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIER2 (RC207065, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIER2.
WB Suggested Anti-MIER2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate
WB Suggested Anti-MIER2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504260 | MIER2 antibody |
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| MIER2 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7) | |||||||
 |
Mouse | IgG1 | OTI3G7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA504403 | MIER2 antibody |
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| MIER2 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1) | |||||||
 |
Mouse | IgG1 | OTI3A1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP52697PU-N | MIER2 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY413697 | MIER2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208612L2V | Lenti ORF particles, Mier2 (GFP-tagged) - Mouse mesoderm induction early response 1, family member 2 (Mier2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR208612L1V | Lenti ORF particles, Mier2 (Myc-DDK-tagged) - Mouse mesoderm induction early response 1, family member 2 (Mier2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||