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MIF antibody

Principal name

MIF antibody

Alternative names for MIF antibody

Macrophage migration inhibitory factor, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF, MMIF

SwissProt ID

P14174 (Human), P30904 (Rat), P34884 (Mouse), P80177 (Bovin), P80928 (Pig), Q1ZZU7 (Sheep)

Gene ID

4282 (MIF), 103694877 (LOC103694877), 17319 (Mif), 81683

Ncbi ID

NP_002406.1, NP_112313.1, 9606, NP_002406, NP_034928, NP_034928.1, NM_010798, NM_031051

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Tr (Turkey), Can (Canine)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of MIF antibody

Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/ml to 10 ng/ml of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18-kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.

General readings

Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29

MIF, 1-115 aa Human, His-tagged, Recombinant

SM6013 MIF antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast.

ICC/IF analysis of MIF in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1/100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of MIF in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1/100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow cytometry analysis of MIF in HeLa cell line, staining at 2-5ug for 1x10⁶cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Western blot analysis: The cell lysates (40 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: Jurkat cell lysate.
Lane 2: THP-1 cell lysate.
Lane 3: HeLa cell lysate.
Lane 4: U937 cell lysate.

Western blot analysis: The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human MIF antibody (1/1,000). Protein wasvisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunoperoxidase of monoclonal antibody to MIF on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma tissue. [antibody concentration sup. ug/ml]

H00004282-M01

Western Blot analysis of MIF expression in transfected 293T cell line by MIF monoclonal antibody (M01), clone 2A10-4D3.Lane 1: MIF transfected lysate(12.5 KDa).Lane 2: Non-transfected lysate.

H00004282-M01

Detection limit for recombinant GST tagged MIF is approximately 0.1ng/ml as a capture antibody.

H00004282-M01

MIF polyclonal antibody ( Cat # PAB6796 ) ( 0.03 µg/ml ) staining of human thymus lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6796

Western blot analysis of HL - 60 extract was resolved by SDS - PAGE , transferred to PVDF membrane and probed with MIF monoclonal antibody , clone 4E4 ( 1 : 1000 ) ( Cat # MAB1071 ) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.

MAB1071

MIF MaxPab rabbit polyclonal antibody. Western Blot analysis of MIF expression in human kidney.

H00004282-D01P

Western Blot analysis of MIF expression in transfected 293T cell line (H00004282-T01) by MIF MaxPab polyclonal antibody.Lane 1: MIF transfected lysate(12.50 KDa).Lane 2: Non-transfected lysate.

H00004282-D01P

MIF MaxPab rabbit polyclonal antibody. Western Blot analysis of MIF expression in Jurkat.

H00004282-D01P

Immunohistochemical analysis of paraffin-embedded rat kidney sections, stain MIF in cytoplasm DAB chromogenic reaction.

PAB7990

Western blot analysis of MIF expression in lysates from Jurkat ( Lane 1, 2 ) , NIH/3T3 ( Lane 3 ) and rat kidney ( Lane 4 ) .

PAB8836

Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) using MIF Antibody

AP08883PU-N

MIF Antibody (0.01µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP08883PU-N

Human Heart; WB Suggested Anti-MIF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; MIF antibody - middle region (ARP56347_P050) in Human Heart cells using Western Blot

ARP56347_P050

Immunohistochemical analysis of paraffin embedded rat kidney sections, staining MIF in cytoplasm, DAB chromogenic reaction

AP23265PU-N

Western blot analysis of Mammary cancer tissue lysis using MIF antibody

AP23265PU-N

Representative Standard Curve

CEK1271

Representative Standard Curve

CEK1272

TP305106

TA303028 (0.03µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303028

TA303028 (0.01µg/ml) staining of Daudi (A) and Jurkat (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303028

Western blot analysis of MIF expression in lysates from Jurkat (Lane 1, 2), 3T3 (Lane 3) and rat kidney (Lane 4).

TA319100

WB analysis of Immunochemical's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa.

TA319159

Western blot analysis of extracts of Jurkat cell lines, using MIF antibody.

TA327040

Immunohistochemistry of paraffin-embedded mouse lung using MIF antibody at dilution of 1:100 (400x lens).

TA327040

WB Suggested Anti-MIF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart

TA337663

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA303028	MIF (C-term) antibody
	Goat Polyclonal Antibody against MIF
	Goat		Aff - Purified	Hu, Ms, Rt	E, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
+1 additional image
TA319100	MIF antibody
	Rabbit polyclonal anti-macrophage migration inhibitory factor (MIF) antibody
	Rabbit		Purified	Ms, Rt	IP, WB	0.1 mg / €355.00
						OriGene Technologies, Inc.
TA319159	MIF antibody
	Chicken polyclonal anti-MIF antibody
$WB analysis of Immunochemical's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa.$	Chicken		Purified	Hu	E, WB	0.5 mg / €590.00
						OriGene Technologies, Inc.
TA327040	MIF antibody
	Rabbit anti-MIF Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / €335.00
						OriGene Technologies, Inc.
+1 additional image
AP08883PU-N	MIF (103-115) antibody
	Goat	IgG	Aff - Purified	Elephant, Hu, Mky, Platypus	E, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
+1 additional image
AP23265PU-N	MIF (C-term) antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €390.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP305106	MIF
	Recombinant protein of human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.

TP723300	MIF
	Purified recombinant protein of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF).
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		25 µg / €230.00
					OriGene Technologies, Inc.

SA6053	MIF (1-115, His-tag)
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.1 mg / €250.00
					OriGene Technologies GmbH
+1 additional image
SA6053X	MIF (1-115, His-tag)
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.5 mg / €600.00
					OriGene Technologies GmbH
+1 additional image
PR27042-5	MIF
	Human	Purified	> 95 %	E. coli	5 µg / €190.00
					Neuromics Antibodies
PR27042-25	MIF
	Human	Purified	> 95 %	E. coli	25 µg / €290.00
					Neuromics Antibodies

Mouse Lenti ORF Particles

Catalog No.
MR200478L2V	Lenti ORF particles, Mif (GFP-tagged) - Mouse macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR200478L1V	Lenti ORF particles, Mif (Myc-DDK-tagged) - Mouse macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR212389L2V	Lenti ORF particles, Mif (GFP-tagged ORF) - Rat macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR212389L1V	Lenti ORF particles, Mif (Myc-DDK-tagged ORF) - Rat macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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MIF antibody

Principal name

Alternative names for MIF antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of MIF antibody

General readings

Primary Antibodies

MIF (C-term) antibody

MIF antibody

MIF antibody

MIF antibody

MIF (103-115) antibody

MIF (C-term) antibody

Proteins & Growth Factors

MIF

MIF

MIF (1-115, His-tag)

MIF (1-115, His-tag)

MIF

MIF

Mouse Lenti ORF Particles

Lenti ORF particles, Mif (GFP-tagged) - Mouse macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mif (Myc-DDK-tagged) - Mouse macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL

Rat Lenti ORF Particles

Lenti ORF particles, Mif (GFP-tagged ORF) - Rat macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mif (Myc-DDK-tagged ORF) - Rat macrophage migration inhibitory factor (Mif), 200 uL, >10^7 TU/mL

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